AFLP primery a jejich kombinace
|
MseI primer |
|||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||||
| CAA | CAC | CAG | CAT | CTA | CTC | CTG | CTT | ||||
| EcoRI primer | Y | NED | AAC | Y1 | Y2 | Y3 | Y4 | Y5 | Y6 | Y7 | Y8 |
| G | HEX | AAG | G1 | G2 | G3 | G4 | G5 | G6 | G7 | G8 | |
| B | FAM | ACA | B1 | B2 | B3 | B4 | B5 | B6 | B7 | B8 | |
| Y | NED | ACC | Y9 | Y10 | Y11 | Y12 | Y13 | Y14 | Y15 | Y16 | |
| G | HEX | ACG | G9 | G10 | G11 | G12 | G13 | G14 | G15 | G16 | |
| B | FAM | ACT | B9 | B10 | B11 | B12 | B13 | B14 | B15 | B16 | |
| Y | NED | AGC | Y17 | Y18 | Y19 | Y20 | Y21 | Y22 | Y23 | Y24 | |
| G | HEX | AGG | G17 | G18 | G19 | G20 | G21 | G22 | G23 | G24 | |
| B | FAM | ATC | B17 | B18 | B19 | B20 | B21 | B22 | B23 | B24 | |
| sekvence primerů | |
| MseI primer | 5´-GAT GAG TCC TGA GTA A xxx-3´ |
| EcoRI primer | 5´-GAC TGC GTA CCA ATT C xxx-3´ |
| jednotlivé sekvence | |||||||||
| EcoRI-AAC (NED) | GACTGCGTACCAATTCAAC | MseI-CAA | GATGAGTCCTGAGTAACAA | ||||||
| EcoRI-AAG (HEX) | GACTGCGTACCAATTCAAG | MseI-CAC | GATGAGTCCTGAGTAACAC | ||||||
| EcoRI-ACA (FAM) | GACTGCGTACCAATTCACA | MseI-CAG | GATGAGTCCTGAGTAACAG | ||||||
| EcoRI-ACC (NED) | GACTGCGTACCAATTCACC | MseI-CAT | GATGAGTCCTGAGTAACAT | ||||||
| EcoRI-ACG (HEX) | GACTGCGTACCAATTCACG | MseI-CTA | GATGAGTCCTGAGTAACTA | ||||||
| EcoRI-ACT (FAM) | GACTGCGTACCAATTCACT | MseI-CTC | GATGAGTCCTGAGTAACTC | ||||||
| EcoRI-AGC (NED) | GACTGCGTACCAATTCAGC | MseI-CTG | GATGAGTCCTGAGTAACTG | ||||||
| EcoRI-AGG (HEX) | GACTGCGTACCAATTCAGG | MseI-CTT | GATGAGTCCTGAGTAACTT | ||||||
| EcoRI-ATC (FAM) | GACTGCGTACCAATTCATC | ||||||||
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