16. 4. 2024

Recent Advances in Plant Hormone Profiling Methods

prof. Mgr. Ondřej Novák, Ph.D.

(Laboratory of Growth Regulators UPOL & Institute of Experimental Botany, CAS)

Připojte se online: meet.google.com/qhf-etyu-sna

Phytohormones play crucial roles in influencing various physiological processes. Whilst metabolism provides the building blocks for plant growth and development, phytohormonal groups are essential for controlling the rate of growth of individual plant parts and for integrating the activities of those parts. High-resolution measurements of phytohormones are therefore necessary for physiological studies of their mode of action. We have developed several fast chromatographic separations and highly sensitive tandem mass spectrometry (LC-MS/MS) methods for simultaneous profiling of phytohormone metabolites. We also focused on efficient cell and organelle isolation, combining different approaches such as density gradient ultracentrifugation or fluorescence-assisted cell/organelle sorting (FACS/FAOS) with a simple one-step purification protocol based on in-tip micro-solid phase extraction and a class-specific miniaturized immunoaffinity chromatography method. We have also recently applied desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) in high-throughput visualisations of multiple phytohormone species and synthetic phytohormone derivatives from plant samples. New analytical tools provide a comprehensive insight into plant hormone regulatory networks, such as the detailed distribution of plant hormones in specific tissues, cells and organelles. Our preliminary data point out to the fact that phytohormone profiles in the plant cell are quite complex and include not only the expected active molecules but also other key representatives that covers phytohormone biosynthesis, conjugation and degradation. By employing these novel methods, we are able to gain a much better understanding of how genetic and experimental manipulations affect plant hormone levels, which will foster a more complete understanding of how these hormones act.

Annotation photo legend: Analytical workflow of phytohormone profiling (Chao Zhang, LGR)

CV of the speaker HERE.