Structure-functional proteomics of biomolecules
News
Instalation of mikroHPLC Agilent Inf1290 (2019/10/21-23)
Successful repair of qTOF MS maXis PLUS turbopump (2019/10/11)
Roman receive GAUK project/financial support for azurin structure/functional study :-)
Tomáš is membre of new GAČR project in cooperation with KACh (Dr. Petr Kozlík)
Successful movo of ESI/APCI-qTOF MS maXis PLUS to joint lab with KACh (2018/11/14-16)
Contact
Katedra Biochemistry Faculty of Science Charles University
Hlavova 2030/8, 1. floor, door Num. 216
Phone: +420 221 951 281
E-mail: miroslav.sulc (at) natur.cuni.cz
People
Miroslav Šulc – Head
Tomáš Ječmen – junior member
Adriana Osičková – partial junior member
Renata Ptáčková – partial junior member
Aleš Ďuriš – Lab manager
Vladimír Ondruška – ext. PhD. student
Roman Tuzhilkin – Mgr. student
Kristýna Barvíková – Mgr. student (Cooperation with Dr. V. Vlachovou, FGU)
Anna Rychlá – Bc. student
Albert Málek – Bc. student
Terka – high school studena (SOČ)
GOALs
Strukture-functional mass spectrometry (MS)
– The development of novel techniques
– Photo-Induced Cross-Linking
– Photo-Induced Electron Release
* Cupredoxin – azurin (P. aeruginosa)
– Clinically relevant protein-protein interactions
MFO system – metabolism of xenobiotics
– interaction of membrane proteins (cytochrom b5, cytochrom P450, and their reduktases)
Bakterial pathogenesis
– Interaction of CyaA toxinu (B. pertusis)
– Interaction of Azurinu (P. aeruginosa)
Interaction of regulatory proteins
* eukaryotic14-3-3
* human calmodulin (CALcium MODULated protein)
Publications
2018
Osickova A, Balashova N, Masin J, Sulc M, Roderova J, Wald T, Brown AC, Koufos E, Chang EH, Giannakakis A, Lally ET, Osicka R.; Cytotoxic activity of Kingella kingae RtxA toxin depends on post-translational acylation of lysine residues and cholesterol binding. Emerg Microbes Infect. 2018 Nov 7;7(1):178. doi: 10.1038/s41426-018-0179-x.
Zahradník J, Kolenko P, Palyzová A, Černý J, Kolářová L, Kyslíková E, Marešová H, Grulich M, Nunvar J, Šulc M, Kyslík P, Schneider B.; The crystal structure of XdpB, the bacterial old yellow enzyme, in an FMN-free form. PLoS One. 2018 Apr 9;13(4):e0195299. doi: 10.1371/journal.pone.0195299.
2016
Šulc, M.; Indra, R.; Moserová, M.; Schmeiser, H.; Frei, E.; Arlt, V.; Stiborová, M.; The impact of individual cytochrome P450 enzymes on oxidative metabolism of benzo[a]pyrene in human livers. Environmental and Molecular Mutagenesis, 2016, Roč. 57, č. 3, s.229-235. IF 2.63 DOI: 10.1002/em.22001
Mrízová, I.; Moserová, M.; Milichovský, J.; Šulc, M.; Kizek, R.; Kopečková, K.; Arlt, V.; Stiborová, M.; Heterologous expression of human cytochrome P450 2S1 in Escherichia coli and investigation of its role in metabolism of benzo[a]pyrene and ellipticine. Monatshefte für Chemie, 2016, Roč. 147, č. 5, s.881-888. DOI: 10.1007/s00706-016-1738-2
Stiborová, M.; Indra, R.; Moserová, M.; Šulc, M.; Hodek, P.; Frei, E.; Schmeiser, H.; Arlt, V.; NADPH- and NADH-dependent metabolism of and DNA adduct formation by benzo[a]pyrene catalyzed with rat hepatic microsomes and cytochrome P450 1A1. Monatshefte für Chemie, 2016, Roč. 147, č. 5, s.847-855. DOI: 10.1007/s00706-016-1713-y
Wald, T.; Osičková, A.; Mašín, J.; Matyska Lišková, P.; Petry-Podgorska, I.; Matoušek, T.; Šebo, P.; Osička, R.; Transmembrane segments of complement receptor 3 do not participate in cytotoxic activities but determine receptor structure required for action of Bordetella adenylate cyclase toxin. Pathogens and Disease, 2016, Roč. 74, č. 3, s.nestránkováno. DOI: 10.1093/femspd/ftw008
Mašín, J.; Osičková, A.; Sukova, A.; Fišer, R.; Halada, P.; Bumba, L.; Linhartova, I.; Osička, R.; Šebo, P.; Negatively charged residues of the segment linking the enzyme and cytolysin moieties restrict the membrane-permeabilizing capacity of adenylate cyclase toxin. Scientific Reports, 2016, Roč. 6, č. SEP 1 2016, s.1-14. DOI: 10.1038/srep29137
Jirků, M.; Lánský, Z.; Bednárová, L.; Šulc, M.; Monincová, L.; Majer, P.; Vyklický, L.; Vondrášek, J.; Teisinger, J.; Boušová, K.; The characterization of a novel S100A1 binding site in the N-terminus of TRPM1. International Journal of Biochemistry and Cell Biology, 2016, Roč. 78, č. September 2016, s.186-193. DOI: 10.1016/j.biocel.2016.07.014
Maňásková-Postlerová, P.; Cozlová, N.; Dorosh, A.; Šulc, M.; Guyonnet, B.; Jonáková, V.; Acrosin inhibitor detection along the boar epididymis. International Journal of Biological Macromolecules, 2016, Roč. 82, č. January 2016, s.733-739. DOI: 10.1016/j.ijbiomac.2015.10.034
Šulc, M.; Mrízová, I.; Černá, T.; Frei, E.; Eckschlager, T.; Adam, V.; Kopečková, K.; Stiborová, M.; Effectiveness of human cytochrome P450 3A4 present in liposomal and microsomal nanoparticles in formation of covalent DNA adducts by ellipticine. Neuroendocrinology Letters, 2016, Roč. 37, č. Suppl. 1, s.95-102.
2015
Ječmen T, Ptáčková R, Černá V, Dračínská H, Hodek P, Stiborová M, Hudeček J, Šulc M.; Photo-initiated crosslinking extends mapping of the protein-protein interface to membrane-embedded portions of cytochromes P450 2B4 and b₅. Methods. 2015 Nov 1;89:128-37. doi: 10.1016/j.ymeth.2015.07.015.
Vimberg V, Kuzma M, Stodůlková E, Novák P, Bednárová L, Šulc M, Gažák R.; Hydnocarpin-Type Flavonolignans: Semisynthesis and Inhibitory Effects on Staphylococcus aureus Biofilm Formation. J Nat Prod. 2015 Aug 28;78(8):2095-103. doi: 10.1021/acs.jnatprod.5b00430.
Bousova K, Jirku M, Bumba L, Bednarova L, Sulc M, Franek M, Vyklicky L, Vondrasek J, Teisinger J.; PIP2 and PIP3 interact with N-terminus region of TRPM4 channel. Biophys Chem. 2015 Oct;205:24-32. doi: 10.1016/j.bpc.2015.06.004.
Zigo M, Dorosh A, Pohlová A, Jonáková V, Šulc M, Maňásková-Postlerová P.; Panel of monoclonal antibodies to sperm surface proteins as a tool for monitoring localization and identification of sperm-zona pellucida receptors. Cell Tissue Res. 2015 Mar;359(3):895-908. doi: 10.1007/s00441-014-2072-9.
Partnerships
Academic Labs
Proteome Centre, UVIC, Canada
Texas Medical School, University of Texas, Houston, USA
School Of Biological & Chemical Sciences, Queen Mary University of London, UK
Institute of Fyzical chemistry J. Heyrovského AV ČR, Praha
Institute of Microbiology AV ČR, Praha
BIOCEV, Praha
Industry
Zentiva, a.s., CZ (2013–2014)
BEDO, Thailand, (2015-2016)
Teaching (Dept. of Biochemistry Charles Univ.)
MC250P51 – Applied biochemistry (LS, time requirement 2/0, 3 credits)
MC250P70 – Life – molekules and Biochemistry (ZS, rtime requirement 2/1, 3 credits)
MC250P08 – Biochemistry and biology of microorganisms (LS, time requirements 2/0, 3 credits)
MC250P09A – Biochemical Methods (ZS, time requirement 2/0, 3 credits)
MC250P09B – Methods for Studies of Biomolecules (ZS, time requirements 2/0, 4 credits)
MC250C09 – Practical Course in Biochemistry and Biology of Microorganisms (LS, time requirements 0/1, 1 credits)
MC250C31N – Practical course in biochemistry
MC250C32N – Practical course in biochemistry for teachers
MC250P40B – Biochemistry II (KATA)
MC250C23 – Biochemistry practise
MC250C33 – Biochemistry practise for teachers
16 – defended Bachelour Thesis
6 – defended Diploma Thesis
3 – defended PhD Thesis
1 – SOČ práce
Alumni
Dominik Musil
Andrea Tichá
Zina Briki
David Landl
Radek Žídek
Kristýna Beňová
Kateřina Pešlová/Haladová
Martina Mazurová
Jana Štrohalmová
Antonín Knížek
Jiří Zahradník
Jana Michalová/Linhartová
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