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Laboratory of Developmental Biology

Head: doc. Ing. RNDr. Vladimir Krylov, Ph.D., RNDr. Tereza Tlapakova, Ph.D.

In an explosively developing field, the laboratory focused on a genetic and genomic approach to embryology in Xenopus laevis and Xenopus tropicalis. In our laboratory we established a culture of Sertoli cell progenitors from the testes of Xenopus tropicalis, in which we study their regenerative potential after injury of juvenile and adult tissues (tadpole tail and adult heart). In cooperation with the Laboratory of Immunoregulations (Assoc. prof. Magdalena Krulová), we also focus on their immunomodulatory abilities towards suppression of the inflammatory response and induction of regenerative processes. We also study the differentiation potential of these stem cells and the ability to form 3D organoids of seminiferous tubules in vitro. Using fluorescence in situ hybridization (FISH), we try to contribute to the knowledge of chromosomal evolution in African pipid frogs employing the diploid species Xenopus tropicalis and polyploid species of the genera Silurana and Xenopus. Last but not least, our group (in cooperation with the Institute of Rheumatology at the 1st Faculty of Medicine) also studies uric acid transport proteins and the influence of human allelic variants on their function.


 

Karyotype and tyramide FISH of Xenopus tropicalis chromosomes.

C:\Users\vavraji1\Downloads\Dif_SC.JPG

Adipogenic, chondrogenic and osteogenic diferentiation of mice Sertolli cells (mSCs).

C:\Users\vavraji1\Downloads\image.png

Microinjection of  XTSSC RFP cells into dorsal vein of X.tropicalis tadpole. 

 

https://www.natur.cuni.cz/biologie/bunecna-biologie/soubory/Labvyvojovebiologie.PNG/image_full


Laboratory: Vinicna 7, ground floor left, No.40 and 45

 

Head of the laboratory:

Krylov Vladimir (Doc.Ing. RNDr., Ph.D.) – (PI) 

V7/P40; +420 22195 1773; vkrylov@natur.cuni.cz

Tlapakova Tereza (RNDr.,Ph.D) (Co-PI) 

V7/P40; +420 22195 1773; tereza.tlapakova@natur.cuni.cz

 

Postdocs:

Knytl Martin (Ing., Ph.D.)

V7/P40; +420 22195 1773; martin.knytl@natur.cuni.cz

PhD students:

Fornaini Nicola Reinaldo (Dr.)

V7/P45; +420 22195 1798; fornainni@natur.cuni.cz

Vávra Jiří (Ing. Mgr.)

V7/P40, +420 22195 1773, vavraji1@natur.cuni.cz

Vegrichtova Marketa (Mgr.)

V7/P45, +420 22195 1798, marketa.vegrichtova@natur.cuni.cz

Zhao Qing (MSc.)

V7/P45; +420 22195 1798; qing.zhao@natur.cuni.cz

 

MSc students:

Hlaviznova Michaela (Bc.) - Email: michaela.cervenkova@natur.cuni.cz

Mertova Irem (Bc.) - Email: irem.mertova@natur.cuni.cz

Pacalova Eliska (Bc.) - Email: eliska.pacalova@natur.cuni.cz

Slovakova Lucie (Bc.) - Email: lucie.slovakova@natur.cuni.cz

Vrzackova Aneta (Bc.) - Email: aneta.vrzackova@natur.cuni.cz

Zahradnikova Hana (Bc.) - Email: hana.zahradnikova@natur.cuni.cz

Zabkova Svetlana (Bc.) - Email: svetlana.zabkova@natur.cuni.cz

 

Technicians:

Vávra Jiří (Ing. Mgr.) - Kontaktní údaje: V7/P40, +420 22195 1773, vavraji1@natur.cuni.cz


Publications 2021:

Song XY, Furman BLS, Premachandra T, Knytl M, Cauret CMS, Wasonga DV, Measey J, Dworkin I, Evans BJ (in press) Sex chromosome degeneration, turnover, and sex-biased expression of sex-linked transcripts in African clawed frogs (Xenopus) Philos Trans R Soc B-Biol Sci. DOI: 10.1098/rstb.2020.0095. IF2019 = 5.680.

 

Targueta CP, Krylov V, Nondilo TE, Lima J, Lourenço LB (2021) Sex chromosome evolution in frogs-helpful insights from chromosome painting in the genus Engystomops. Heredity 126(3):396-409. DOI: 10.1038/s41437-020-00385-7. IF2019 = 3.436.

Publications 2020:

Sember A, Pelikanova S, de Bello Cioffi M, Slechtova V, Hatanaka T, Do Doan H, Knytl M, Rab P (2020) Taxonomic diversity not associated with gross karyotype differentiation: The case of bighead carps, genus Hypophthalmichthys (Teleostei, Cypriniformes, Xenocyprididae). Genes 11(5):479 IF2019 = 3.759.

Cauret CMS, Gansauge MT, Tupper AS, Furman BLS, Knytl M, Song XY, Greenbaum E, Meyer M, Evans BJ (2020) Developmental systems drift and the drivers of sex chromosome evolution. Mol Biol Evol 37(3):799-810. IF2019 = 11.062.

Furman BLS, Cauret CMS, Knytl M, Song XY, Pramachandra T, Ofori-Boateeng C, Jordan DC, Horb ME, Evans BJ (2020) A frog with three sex chromosomes that co-mingle together in nature: Xenopus tropicalis has a degenerate W and a Y that evolved from a Z chromosome. PLoS Genet. PLoS Genet 16(11): e1009121. IF2019 = 5.174.

Svaton M, Skvarova Kramarzova K, Kanderova V, Mancikova A, Smisek P, Jesina P, Krijt J, Stiburkova B, Dobrovolny R, Sokolova J, Bakardjieva-Mihaylova V, Vodickova E, Rackova M, Stuchly J, Kalina T, Stary J, Trka J, Fronkova E, Kozich V (2020). A homozygous deletion in the SLC19A1 gene as a cause of folate-dependent recurrent megaloblastic anemia. Blood. 135(26):2427-2431. DOI: 10.1182/blood.2019003178. IF2019 = 17.794.

Publikace 2019:

Nguyen TXMVegrichtova MTlapakova T, Krulova M, Krylov V. (2019) Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells. Stem Cells Int 5, 8387478 (IF 3.989).

Nguyen TXMVegrichtova MTlapakova T, Krulova M, Krylov V. (2019) The interconnection between cytokeratin and cell membrane-bound β-catenin in Sertoli cells derived from juvenile Xenopus tropicalis testes. Biology open, submitted (IF 2.217).

Stiburkova B, Bohata J, Minarikova I, Mancikova AVavra JKrylov V, Doležel Z. (2019) Clinical and Functional Characterization of a Novel URAT1 Dysfunctional Variant in a Pediatric Patient with Renal Hypouricemia. Appl Sci 9, 3479 (IF 2,217).

Toyoda Y, Mančíková AKrylov V, Morimoto K, Pavelcová K, Bohatá J, Pavelka K, Pavlíková M, Suzuki H, Matsuo H, Takada T, Stiburkova B. (2019) Functional Characterization of Clinically-Relevant Rare Variants in ABCG2 Identified in a Gout and Hyperuricemia Cohort. Cells 8(4) (IF 4.829)

Seroussi E, Knytl M, Pitel F, Elleder D, Krylov V, Leroux S, Morisson M, Yosefi S, Miyara S, Ganesan S, Ruzal M, Andersson L, Friedman-Einat M. (2019) Avian expression patterns and genomic mapping implicate leptin in digestion and TNF in immunity suggesting that their interacting adipokine role has been acquired only in mammals. Int J Mol Sci 20, 4489 (IF = 4,183).

Petelak A.Krylov V. (2019) Surface sperm cell ubiquitination directly impaired blastocyst formation rate after intracytoplasmic sperm injection in pig. Theriogenology 135, 115-120 (IF 2.136).

Targueta CP, Krylov V, Nondilo TE, Lima J,  Lourenço LB. Sex chromosome evolution in frogs – helpful insights from chromosome painting in the genus Engystomops. Heredity, submitted. (IF 3.872).

 

 

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