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Chromatographic analytical methods

Assoc. Prof. Zuzana Bosáková, Ph.D.
(E-mail: bosakova@natur.cuni.cz)


Prof. Pavel Coufal, Ph.D. (Head of the Team)
(E-mail: pcoufal@natur.cuni.cz)

  • microseparation methods
  • liquid chromatography
  • capillary electrophoresis – mass spectrometry


Assoc. Prof. Radomír Čabala, Ph.D.
(E-mail: cabala@natur.cuni.cz)

  • derivatization methods for gas chromatography
  • gas and liquid chromatography
  • mass spectrometry


Petr Kozlík, MSc.
(E-mail: petr.kozlik@natur.cuni.cz)

  • mass spectrometry
  • mass spectrometry connected to separation techniques (HPLC-MS)
  • characterization of separation systems
  • development and validation of separation methods
  • forced degradation studies and stability testing of active pharmaceutical ingredients and dosage forms


Tomáš Křížek, Ph.D.
(E-mail: krizek@natur.cuni.cz)

  • analysis of biological samples using nano-HPLC-MS/MS on chip and capillary electrophoresis
  • development of method for determination of coenzymes in plant extracts
  • development of electrophoretic determination of substrates a products of enzymes glycosidase
  • development of contactless conductivity detection methods


Anna Kubíčková, Ph.D.
(E-mail: anna.kubickova@natur.cuni.cz)

  • HPLC separations of ligands for biomedicine application of DOTA derivatives
  • application of new generation columns (e.g. Purospher STAR or ZIC-HILIC columns specially destined for polar substances)
  • development of postcolumn derivatization methods based on forming of complexes


Karel Nesměrák, Ph.D.
(E-mail: nesmerak@natur.cuni.cz)

  • analytical determination of pharmaceutically and biologically important substances
  • study of relationships between structure and properties of compounds
  • electrochemical and spectrometric methods for study of physical-chemical properties of compounds


Jana Sobotníková (Suchánková), Ph.D.
(E-mail: jana.sobotnikova@natur.cuni.cz)

  • high performance liquid chromatography, new stationary phases
  • capillary liquid chromatography, capillary monolithic and packing columns for HPLC-MS
  • separation of biologically active peptides



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