Novel approach to the construction of nucleic acids-peptides conjugates
Scientists from the Joint Laboratory of Bioorganic and Medicinal Chemistry of Nucleic Acids of the IOCB ASCR and the Faculty of Science of the CU (group of Assoc. Prof. Hocek) have published a new synthetic method for the bioconjugation of nucleic acids with peptides. It consists in the synthesis of formylaryl-derivátives of nucleoside triphosphates, their enzymatic incorporation to DNA by polymerase followed by reductive amination with lysin-containing peptides. This method paves the way for applications in medicinal chemistry (drug delivery) and chemical biology (e.g. cross-linking of DNA with binding proteins). The work has just been published in prestigious journal Chemistry - A European Journal.
Raindlová, V.; Pohl, R.; Hocek, M.: "Synthesis of aldehyde-linked nucleotides and DNA and their bioconjugations with lysine and peptides through reductive amination" Chem. Eur. J. 2012, 18, 4080–4087.