Workshop: Advanced Methods of Scanning Electron Microscopy
This course is focused on a theoretical and practical introduction to scanning electron microscopy and its biological applications. The programme covers fundamental principles of SEM, current methods of specimen preparation, including cryo approaches, methods of volume SEM like FIB SEM, SBF SEM or MAT, and SEM applications in immunolocalization or correlative microscopy. The main goal of the practical part is to familiarise participants with microscope operation, image acquisition, data processing and useful tricks and tips in sample preparation. The course is limited to 15 participants including both begining and intermediate SEM users. This course is supported by the program for large research infrastructures of the Ministry of Education, Youth and Sports within the project “National Infrastructure for Biological and Medical Imaging (Czech-BioImaging)“.
SCHEDULE OF THE WORKSHOP
Day 1
(Location: VMCF Viničná, Microscopy Center, Viničná 7, Praha 2)
10:00 – 10:10 Welcome Address (Miroslav Hyliš, Faculty of Science, Charles University, Praha)
10:00 – 10:55 Principles of Scanning Electron Microscopy (Vladislav Krzyžánek, Institute of Scientific Instruments, CAS, Brno)
10:55 – 12:00 Overview of SEM Sample Preparation Methods at RT and Cryo- conditions (Jana Nebesářová, Faculty of Science, Charles University, Praha)
12:00 – 13:00 Lunch
13:00 – Practicals
Day 2
(Location: VMCF Viničná, Microscopy Center, Viničná 7, Praha 2)
09:00 – 10:15 SBF SEM and Micro Array Tomography (Jiří Týč, Biology Centre, CAS, České Budějovice)
10:15 – 11:00 FIB SEM in Biology Research (Markéta Dalecká, BIOCEV, Vestec)
11:00 – 11:30 Coffee break
11:30 – 12:00 The Use of SEM in correlative microscopy (Marie Vancová, Biology Centre, CAS, České Budějovice)
12:00 – 13:00 Lunch
13:00 – 18:30 Practicals
18:30 – Course Dinner
Day 3
(Location: VMCF Viničná, Microscopy Center, Viničná 7, Praha 2)
09:00 – 10:00 Introduction in Image Processing (Martin Schätz, Faculty of Science, Charles Universíty , Praha)
10:00 – 11:00 How to do 3D reconstruction from SEM images? (Jiří Týč, František Kitzberger, Biology Centre, CAS, České Budějovice)
11:00 – 11:30 Coffee break
11:30 – 12:00 Methods of immunolocalization in SEM (Jana Nebesářová, Faculty of Science, Charles Universíty, Praha)
12:00 – 13:00 Lunch
13:00 – 16:30 Practicals
16:30 The end of the course
Registration fees:
CSMS member: 4000 CZK
CSMS non-member: 5000 CZK
Industry: 8000 CZK
The course fee includes admission, course materials, lunches and coffee breaks
Registration fees: Contact:
CSMS member: 4000 CZK Petra Hůle
CSMS non-member: 5000 CZK E-mail: hulep@natur.cuni.cz,
Industry: 8000 CZK Phone:+420733461169
The course fee includes admission, course materials, lunchesand coffee breaks
Registration:
https://app.greception.com/form-login-window/1e426311/
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