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Seminar of the Faculty of Science at BIOCEV – ​”Cancer immunotherapy and tumor models” by RNDr. Michal Šmahel, Ph.D.

Dear colleagues, we would like to draw your attention to the next seminar of the Faculty of Science at BIOCEV -Tuesday 28th of June 2016 at 3:00 p.m. in the main BIOCEV building, Průmyslová 595, Vestec, conference hall number U2.012 (2nd floor).


RNDr. Michal Šmahel, Ph.D.: ​Cancer immunotherapy and tumor models

Cancer immunotherapy achieved great progress in clinical trials in recent years but still most patients do not respond to this treatment. In our lab, we endeavor to enhance the effect of cancer immunotherapy by the combination of various immunostimulatory methods applied against mouse tumors with downregulation of MHC class I expression which represents one of the most frequent tumor escape mechanisms. To immunize against tumors, we constructed plasmids carrying various fusion genes. These DNA vaccines, that are delivered by a gene gun, are mainly aimed against the human papillomavirus E7 oncoprotein. Moreover, immunization was also aimed against legumain that is overexpressed in M2-polarized tumor-associated macrophages supporting tumor growth and against markers of cancer stem cells (Sox2 and Aurka). Next, we tried to enhance the antitumor effect of DNA immunization by the depletion of regulatory T cells with antibody against CD25 and by the blockade of the PD-1 immune checkpoint receptor. For the analysis of mechanisms contributing to antitumor immune reactions, we modify tumor cell lines with the CRISPR/Cas9 system.

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Published: Jun 24, 2016 12:45 PM

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