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Laboratory of heme-containing sensor proteins

Contact; People;Research fields; Projects; Papers; Collaboration; Points of Interest; Teaching; Alumni

News

Our review on heme sensor proteins has been accepted (October 26, 2019) - Shimizu T., Lengalova A., Martínek V., Martínková M. (2019) Heme: Emergent roles of heme in signal transduction, functional regulation and as catalytic centres, Chem. Soc. Rev. (Royal Society of Chemistry), in press, IF2017 = 40.443 - https://doi.org/10.1039/c9cs00268e

We published a new paper on diguanylate cyclase (YddV) (September 10, 2019) - https://doi.org/10.1016/j.jinorgbio.2019.110833

Veronika Prošková (Fojtíková) successfully defended her PhD thesis (September 10, 2018)

Jakub Vávra attended the XX International Conference on Oxygen Binding and Sensing Proteins in Barcelona (Spain) (September 3 - 6, 2018)

Markéta Martínková, Alžběta Lengálová and Václav Martínkek attended the 43rd FEBS Congress in Prague (Czech Republic) (July 7-12, 2018)

 

Contact

Department of Biochemistry Faculty of Science, Charles University

Hlavova 2030/8, 1. floor, door number 217

tel: +420 221 951 242

e-mail: marketa.martinkova (at) natur.cuni.cz

 

People

Markéta Martínková – head of the team

Toru Shimizu – member

Václav Martínek – member

Alžběta Lengálová – PhD student

Jakub Vavra – PhD student

Eva Tajovská – undergraduate student

Lukrécie Vojáčková – undergraduate student

Artur Sergunin – undergraduate student

Dominika Andrlová – undergraduate student

Kateřina Čížová – undergraduate student

Anna Ammerova - technician 

 

Research fields

Heme enzymes

- involvement in carcinogenesis

Heme-containing sensor proteins

- structure-function relationships

- involvement in carcinogenesis

- involvement in bacterial pathogenesis - antibacterial therapy

Mechanism of signal transduction in eukaryotic and prokaryotic cells

- role of heme in regulation pathways (for example in regulation of protein expression)

 

Projects

JPIAMR2019-087

Time period: 2020-2022

Title: Prevention of antibiotic resistance by TARGEted Treatment of pneumonia in children (TARGET) (international consortium, principal investigator)

GA ČR, 15-19883S

Time period: 2015-2017

Title: Molecular mechanisms of intraprotein/interdomain signal transduction in model heme sensor proteins

IPSEN Pharma, os.

Time period: 2014-2015

Title: External sponsored study “Comparison of the adsorption capacity and trapping effectiveness of diosmectite and charcoal on the compounds causing the 10 most frequent intoxications in acute medicine”

GA ČR, 203/09/0812

Time period: 2009-2011

Title: Mechanism of modulation of cytochrome P450 1A1/2 mediated oxidation of drugs and carcinogens by cytochrome b5; experimental and computational approach (participation in the project granted to RNDr. Václav Martínek, PhD.)

UNCE 204025/2012

Time period: 2012-2017

Title: Modern technologies for identification and optimization of anti-tumor drugs of the new generation (participation in the project granted to doc. RNDr. Miroslav Šulc, PhD.)

GAUK 756214

Time period: 2014-2015

Title: Molecular mechanisms of intraprotein/interdomain signal transduction in heme-sensor proteins, namely histidine kinase (AfGcHK) and diguanylate cyclase (YddV)

GAUK 362115

Time period: 2015-2017

Title: Study of the signal transduction in model heme-containing sensor proteins (namely globin-coupled histidine kinase, AfGcHK and phosphodiesterase, EcDOS) aimed at alternation of their enzyme activity

GAUK 704217

Time period:  2017-2019

Title: Structure and function relationship of model heme-containing sensor proteins and their participation in processes associated with human health

 

Papers

2019

Shimizu T., Lengalova A., Martínek V., Martínková M. (2019) Heme: Emergent roles of heme in signal transduction, functional regulation and as catalytic centres, Chem. Soc. Rev. in press https://doi.org/10.1039/c9cs00268e

Lengalova A., Fojtikova-Proskova V, Vavra J, Martínek V, Stranava M, Shimizu T, Martinkova M (2019) Kinetic Analysis of a Globin-Coupled Diguanylate Cyclase, YddV: Effects of Heme Iron Redox State, Axial Ligands, and Heme Distal Mutations on Catalysis, J  Inorg Biochem, 201: 110833.

2018

Stiborová M, Dračínská H, Bořek-Dohalská L, Klusoňová Z, Holecová J, Martínková M, Schmeiser HH, Arlt VM (2018) Exposure to Endocrine Disruptors 17alpha-Ethinylestradiol and Estradiol Influences Cytochrome P450 1A1-Mediated Genotoxicity of Benzo[a]Pyrene and Expression of This Enzyme in Rats, Toxicology400–401: 48–56

2017

Stranava M, Man P, Skálová T, Kolenko P, Blaha J, Fojtikova V, Martínek V, Dohnálek J, Lengalova A, Rosůlek M, Shimizu T, Martinkova M (2017) Coordination and redox state-dependent structural changes of the heme-based oxygen sensor AfGcHK associated with intraprotein signal transduction, J Biol Chem292: 20921-20935

Mináriková M, Fojtikova V, Vyskočilová E, Sedláček J, Šikut M, Borek-Dohalska L, Stiborová M, Martinkova M (2017) The capacity and effectiveness of diosmectite and charcoal in trapping the compounds causing the most frequent intoxications in acute medicine: a comparative study, Environ Toxicol Phar 52: 214-220

2016

Fojtikova V, Bartosova M, Man P, Stranava M, Shimizu T, Martinkova M (2016) Effects of hydrogen sulfide on the heme coordination structure and catalytic activity of the globin-coupled oxygen sensor AfGcHK, Biometals 29: 715-729

Stranava M, Martinek V, Man P, Fojtikova V, Kavan D, Vaněk O, Shimizu T, Martinkova M (2016) Structural Characterization of the Heme-based Oxygen Sensor, AfGcHK, its Interactions with the Cognate Response Regulator, and their Combined Mechanism of Action in a Bacterial Two-component Signaling System. Proteins, 84: 1375-89

Kuipers K, Gallay C, Martínek V, Rohde M, Martínková M, van der Beek SL, Jong WS, Venselaar H, Zomer A, Bootsma H, Veening JW, de Jonge MI (2016) Highly conserved nucleotide phosphatase essential for membrane lipid homeostasis in Streptococcus pneumoniae. Mol Microbiol 101: 12-26

Lambry J-Ch, Stranava M, Lobato L, Martinkova M, Shimizu T, Liebl U, Vos MH (2016) Ultrafast Spectroscopy Evidence for Picosecond Ligand Exchange at the Binding Site of a Heme Protein: Heme-based Sensor YddV. J Phys Chem Lett 7: 69–74

2015

Fojtikova V, Stranava M, Vos M H, Liebl U,  Hraníček J, Kitanishi K, Shimizu T, Martinkova M (2015) Kinetic Analysis of a Globin-coupled Histidine Kinase, AfGcHK: Effects of the Heme Iron Complex, Response Regulator and Metal Cations on Autophosphorylation Activity. Biochemistry 54: 5017–5029

Pavlou A, Martínková M, Shimizu T, Kitanishi K, Stranava M, Loullis A, Pinakoulaki E (2015)  Probing the ligand recognition and discrimination environment of the globin-coupled oxygen sensor protein YddV by FTIR and time-resolved step-scan FTIR spectroscop.Phys Chem Chem Phys 17: 17007-17015

Yan F, Fojtikova V, Man P, Stráňava M, Martínková M, Du Y, Huang D, Shimizu T (2015)  Catalytic Enhancement of the Heme-based Oxygen-Sensing Phosphodiesterase EcDOS by Hydrogen Sulfide Is Caused by Changes in Heme Coordination Structure, BioMetals 28: 637-652

Shimizu T, Yan F, Huang D, Stráňava M, Bartošová M, Fojtíková V, Martínková M (2015) Molecular Characteristics of Heme-based Gas (O2, NO and CO) Sensors. Chem Rev 115: 6491−6533

 

Collaboration

Academic Partners

Laboratory of Pediatric Infect. Diseases, Nijmegen, Netherlands (Marien de Jonge - microbiology)

Ecole Polytechnique, Palaiseau, France (Marten Vos and Ursula Liebl - ultrafast spectroscopy)

Université Montpellier, France (Reinhard Lange - spectroscopy under high pressure)

Cyprus University of Technology, Limessol and University of Cypru, Nicosia, Cyprus (Constantinos Varotsis and Eftychia Pinakoulaki - FTIR and Raman spectroscopy)

Tohoku University, Sendai, Japan (Kazuhiko Igarashi - eukaryotic heme sensor proteins)

Institute of Microbiology, Prague, Czech Republic (Miroslav Šulc - mass spectrometry)

BIOCEV, Vestec, Czech Republic (Petr Man and Petr Pompach - mass spectrometry; Jan Dohnálek - X-ray)

Industry and commercial sphere

PSEN Pharma, os. (France, 2014- 2015)

BEDO (Thailand, 2015-2016)

 

Points of Interest

Presentation card

Web presentation - www.natur.cuni.cz/martinkova

 

Teaching

MC250P19B – Clinical and analytical biochemistry (summer semester, 3/0, 4 E-Credits)

MC250C44 – Practical course in clinical biochemistry (winter semester, 4/0, 6 E-Credits)

6 - Defended Bachelor thesis

3 - Defended M.Sc. thesis

2 - Defended PhD thesis
 

Alumni

Dr. Martin Stráňava – Covance company

Dr. Veronika Prošková (Fojtikova) - private health-care company

 

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