Laboratory of heme-containing sensor proteins
News
Our review on heme sensor proteins has been accepted [October 26, 2019] – Shimizu T., Lengalova A., Martínek V., Martínková M. (2019) Heme: Emergent roles of heme in signal transduction, functional regulation and as catalytic centres, Chem. Soc. Rev. (Royal Society of Chemistry), in press, IF2017 = 40.443 – https://doi.org/10.1039/c9cs00268e
We published a new paper on diguanylate cyclase (YddV) [September 10, 2019] – https://doi.org/10.1016/j.jinorgbio.2019.110833
Veronika Prošková (Fojtíková) successfully defended her PhD thesis [September 10, 2018]
Jakub Vávra attended the XX International Conference on Oxygen Binding and Sensing Proteins in Barcelona (Spain) [September 3 – 6, 2018]
Markéta Martínková, Alžběta Lengálová and Václav Martínkek attended the 43rd FEBS Congress in Prague (Czech Republic) [July 7 – 12, 2018]
Contact
Department of Biochemistry Faculty of Science, Charles University
Hlavova 2030/8, 1. floor, door number 217
tel: +420 221 951 242
e-mail: marketa.martinkova (at) natur.cuni.cz
People
Markéta Martínková – head of the team
Toru Shimizu – member
Václav Martínek – member
Alžběta Lengálová – PhD student
Jakub Vavra – PhD student
Eva Tajovská – undergraduate student
Lukrécie Vojáčková – undergraduate student
Artur Sergunin – undergraduate student
Dominika Andrlová – undergraduate student
Kateřina Čížová – undergraduate student
Anna Ammerova – technician
Research fields
Heme enzymes
– involvement in carcinogenesis
Heme-containing sensor proteins
– structure-function relationships
– involvement in carcinogenesis
– involvement in bacterial pathogenesis – antibacterial therapy
Mechanism of signal transduction in eukaryotic and prokaryotic cells
– role of heme in regulation pathways (for example in regulation of protein expression)
Projects
JPIAMR2019-087
Time period: 2020-2022
Title: Prevention of antibiotic resistance by TARGEted Treatment of pneumonia in children (TARGET) (international consortium, principal investigator)
GA ČR, 15-19883S
Time period: 2015-2017
Title: Molecular mechanisms of intraprotein/interdomain signal transduction in model heme sensor proteins
IPSEN Pharma, os.
Time period: 2014-2015
Title: External sponsored study “Comparison of the adsorption capacity and trapping effectiveness of diosmectite and charcoal on the compounds causing the 10 most frequent intoxications in acute medicine”
GA ČR, 203/09/0812
Time period: 2009-2011
Title: Mechanism of modulation of cytochrome P450 1A1/2 mediated oxidation of drugs and carcinogens by cytochrome b5; experimental and computational approach (participation in the project granted to RNDr. Václav Martínek, PhD.)
UNCE 204025/2012
Time period: 2012-2017
Title: Modern technologies for identification and optimization of anti-tumor drugs of the new generation (participation in the project granted to doc. RNDr. Miroslav Šulc, PhD.)
GAUK 756214
Time period: 2014-2015
Title: Molecular mechanisms of intraprotein/interdomain signal transduction in heme-sensor proteins, namely histidine kinase (AfGcHK) and diguanylate cyclase (YddV)
GAUK 362115
Time period: 2015-2017
Title: Study of the signal transduction in model heme-containing sensor proteins (namely globin-coupled histidine kinase, AfGcHK and phosphodiesterase, EcDOS) aimed at alternation of their enzyme activity
GAUK 704217
Time period: 2017-2019
Title: Structure and function relationship of model heme-containing sensor proteins and their participation in processes associated with human health
Papers
2019
Shimizu T., Lengalova A., Martínek V., Martínková M. (2019) Heme: Emergent roles of heme in signal transduction, functional regulation and as catalytic centres, Chem. Soc. Rev. in press https://doi.org/10.1039/c9cs00268e
Lengalova A., Fojtikova-Proskova V, Vavra J, Martínek V, Stranava M, Shimizu T, Martinkova M (2019) Kinetic Analysis of a Globin-Coupled Diguanylate Cyclase, YddV: Effects of Heme Iron Redox State, Axial Ligands, and Heme Distal Mutations on Catalysis, J Inorg Biochem, 201: 110833.
2018
Stiborová M, Dračínská H, Bořek-Dohalská L, Klusoňová Z, Holecová J, Martínková M, Schmeiser HH, Arlt VM (2018) Exposure to Endocrine Disruptors 17alpha-Ethinylestradiol and Estradiol Influences Cytochrome P450 1A1-Mediated Genotoxicity of Benzo[a]Pyrene and Expression of This Enzyme in Rats, Toxicology, 400–401: 48–56
2017
Stranava M, Man P, Skálová T, Kolenko P, Blaha J, Fojtikova V, Martínek V, Dohnálek J, Lengalova A, Rosůlek M, Shimizu T, Martinkova M (2017) Coordination and redox state-dependent structural changes of the heme-based oxygen sensor AfGcHK associated with intraprotein signal transduction, J Biol Chem, 292: 20921-20935
Mináriková M, Fojtikova V, Vyskočilová E, Sedláček J, Šikut M, Borek-Dohalska L, Stiborová M, Martinkova M (2017) The capacity and effectiveness of diosmectite and charcoal in trapping the compounds causing the most frequent intoxications in acute medicine: a comparative study, Environ Toxicol Phar 52: 214-220
2016
Fojtikova V, Bartosova M, Man P, Stranava M, Shimizu T, Martinkova M (2016) Effects of hydrogen sulfide on the heme coordination structure and catalytic activity of the globin-coupled oxygen sensor AfGcHK, Biometals 29: 715-729
Stranava M, Martinek V, Man P, Fojtikova V, Kavan D, Vaněk O, Shimizu T, Martinkova M (2016) Structural Characterization of the Heme-based Oxygen Sensor, AfGcHK, its Interactions with the Cognate Response Regulator, and their Combined Mechanism of Action in a Bacterial Two-component Signaling System. Proteins, 84: 1375-89
Kuipers K, Gallay C, Martínek V, Rohde M, Martínková M, van der Beek SL, Jong WS, Venselaar H, Zomer A, Bootsma H, Veening JW, de Jonge MI (2016) Highly conserved nucleotide phosphatase essential for membrane lipid homeostasis in Streptococcus pneumoniae. Mol Microbiol 101: 12-26
Lambry J-Ch, Stranava M, Lobato L, Martinkova M, Shimizu T, Liebl U, Vos MH (2016) Ultrafast Spectroscopy Evidence for Picosecond Ligand Exchange at the Binding Site of a Heme Protein: Heme-based Sensor YddV. J Phys Chem Lett 7: 69–74
2015
Fojtikova V, Stranava M, Vos M H, Liebl U, Hraníček J, Kitanishi K, Shimizu T, Martinkova M (2015) Kinetic Analysis of a Globin-coupled Histidine Kinase, AfGcHK: Effects of the Heme Iron Complex, Response Regulator and Metal Cations on Autophosphorylation Activity. Biochemistry 54: 5017–5029
Pavlou A, Martínková M, Shimizu T, Kitanishi K, Stranava M, Loullis A, Pinakoulaki E (2015) Probing the ligand recognition and discrimination environment of the globin-coupled oxygen sensor protein YddV by FTIR and time-resolved step-scan FTIR spectroscop.Phys Chem Chem Phys 17: 17007-17015
Yan F, Fojtikova V, Man P, Stráňava M, Martínková M, Du Y, Huang D, Shimizu T (2015) Catalytic Enhancement of the Heme-based Oxygen-Sensing Phosphodiesterase EcDOS by Hydrogen Sulfide Is Caused by Changes in Heme Coordination Structure, BioMetals 28: 637-652
Shimizu T, Yan F, Huang D, Stráňava M, Bartošová M, Fojtíková V, Martínková M (2015) Molecular Characteristics of Heme-based Gas (O2, NO and CO) Sensors. Chem Rev 115: 6491−6533
Collaboration
Academic Partners
Laboratory of Pediatric Infect. Diseases, Nijmegen, Netherlands (Marien de Jonge – microbiology)
Ecole Polytechnique, Palaiseau, France (Marten Vos and Ursula Liebl – ultrafast spectroscopy)
Université Montpellier, France (Reinhard Lange – spectroscopy under high pressure)
Cyprus University of Technology, Limessol and University of Cypru, Nicosia, Cyprus (Constantinos Varotsis and Eftychia Pinakoulaki – FTIR and Raman spectroscopy)
Tohoku University, Sendai, Japan (Kazuhiko Igarashi – eukaryotic heme sensor proteins)
Institute of Microbiology, Prague, Czech Republic (Miroslav Šulc – mass spectrometry)
BIOCEV, Vestec, Czech Republic (Petr Man and Petr Pompach – mass spectrometry; Jan Dohnálek – X-ray)
Industry and commercial sphere
PSEN Pharma, os. (France, 2014 – 2015)
BEDO (Thailand, 2015 – 2016)
Points of Interest
Web presentation – www.natur.cuni.cz/martinkova
Teaching
MC250P19B – Clinical and analytical biochemistry (summer semester, 3/0, 4 E-Credits)
MC250C44 – Practical course in clinical biochemistry (winter semester, 4/0, 6 E-Credits)
6 – Defended Bachelor thesis
3 – Defended M.Sc. thesis
2 – Defended PhD thesis
Alumni
Dr. Martin Stráňava – Covance company
Dr. Veronika Prošková (Fojtikova) – private health-care company
Document Actions