Laboratory of Structural Biochemistry of Immune Recognition
For news and more information, please visit the full website of our research group Laboratory of Structural Biochemistry of Immune Recognition
Our research group is interested in cell-cell recognition processes, i.e., how the immune cells, for example, NK cells, recognize by receptors expressed on their surface the target proteins expressed on the surface of healthy cells or of the tumour, virally infected or stressed cells. We are interested firstly in the structure-function relationship, using a wide range of modern methods of research of protein structure, their properties and interactions, and secondly, how we could transfer the gained knowledge into a practical level, besides other things by designing artificial protein constructs which would help the immune cells to recognize better and eliminate the target cells.
Contact
Department of Biochemistry, Faculty of Science, Charles University
Hlavova 2030/8, 2nd floor, door No. 204
Tel: +420 221 951 272
E-mail: ondrej.vanek@natur.cuni.cz
People
Ondřej Vaněk – group leader
Jan Bláha – postdoctoral fellow (presently post-doc stay at EMBL Hamburg)
Barbora Kalousková – postdoctoral fellow (presently post-doc stay at TU Wien)
Ondřej Skořepa – postdoctoral fellow
Celeste de Sousa Santos Abreu – Ph.D. student
Shiva Nejadebrahim – Ph.D. student
Tomáš Otépka – MSc. student
Kristýna Bednaříková – MSc. student
Gabriela Chytrá – MSc. student
Maxmilián Hejl – MSc. student
Kristýna Pazderová – MSc. student
Matouš Tulpa – MSc. student
Nadezda Bragina – MSc. student
Yurii Dubanych – MSc. student
Anastassiya Rudenko – MSc. student
Zuzana Urbanová – MSc. student
Jakub Jeníček – Bc. student
Jakub Hubáček – Bc. student
Aims
Structural research of complexes of NK cell receptors with their ligands
– Used methods
– Recombinant protein expression within the HEK293 human cell line
– Biophysical characterization of proteins and their interactions (AUC, ITC, MST, DLS, DSF, SPR)
– Protein crystallography, structural mass spectrometry, SAXS
Research of NK cell receptors arrangement on the surface of a living cell
– Superresolution microscopy, fluorescence microscopy, flow cytometry, single-molecule localization microscopy (SMLM)
Production of fusion constructs of nanobodies with ligands of activation receptors of NK cells and testing their biological activity
– Isolation of NK cells from the blood of healthy donors
– Cytotoxicity tests of NK cells against tumour cell lines
Production of fusion constructs with IL-2 or growth factors
– Expression, purification and study of properties of fusion proteins containing protein hormones or cytokines
Projects
Czech Science Foundation, 15-15181S (O. Vaněk)
Title: Harnessing soluble forms of NK cell receptors and their ligands for the generation of novel anticancer immunotherapeutics
Duration: 2015 – 2017
Grant Agency of the Charles University, 161216 (J. Bláha / B. Kalousková)
Title: Utilization of a structural description of the interaction of human lymphocyte complex NKR-P1 and LLT1 for development of immunoactive fusion therapeutics
Duration: 2016 – 2018
Grant Agency of the Charles University, 927916 (S. Pažický / O. Skořepa)
Title: Impact of NKp30 glycosylation and oligomerization on its complex formation – the key features for NK cells activation
Duration: 2016 – 2018
Ministry of Education, Youth and Sports of the Czech Republic, INTER-COST program, LTC17065 (O. Vaněk)
Title: Utilization of integrative molecular biophysical approaches for the functional studies of human natural killer cell receptors and formation of their complexes with tumour ligands
Duration: 2017 – 2020
Czech Science Foundation, 18-10687S (O. Vaněk)
Title: New ligands for old receptors of human natural killer cells: structure, assembly within the immune synapse and potential for therapy
Duration: 2018 – 2020
Grant Agency of the Charles University, 1740318 (D. Cmunt)
Title: Utilization of activation ligands and nanobodies for directed stimulation of NK cells cytotoxicity
Duration: 2018 – 2019
Grant Agency of the Charles University, 1378219 (B. Kalousková)
Title: Quaternary structure of selected human lymphocyte receptors through the looking glass of super-resolution microscopy: What is the biological reality behind the crystal structures?
Duration: 2019 – 2021
Czech Science Foundation, 20-13029S (O. Vaněk, spoluřešitel)
Title: Novel clinically relevant IL-2 immunotherapeutics for cancer treatment
Duration: 2020 – 2022
Grant Agency of the Charles University, 1436020 (J. Houserová)
Title: Structural biochemistry of NK cell complexes NKp44:PCNA and NKp46:Epa1, and utilization of Epa1 for stimulation of NK cells
Duration: 2020 – 2022
Grant Agency of the Charles University, 1436220 (C. Abreu)
Title: Activation of NK cell anti-tumour response using bispecific fusion proteins with nanobodies
Duration: 2020 – 2022
Ministry of Education, Youth and Sports of the Czech Republic, INTER-COST program, LTC20078 (O. Vaněk)
Title: Glycan-dependent regulation of NK cell recognition: structural biology and novel methods of glycopeptide analysis
Duration: 2020 – 2023
Grant Agency of the Charles University, 318122 (K. Pazderová)
Title: Activation receptor NKp80 and its ligand AICL: structure and mutual interaction in solution and on the surface of human NK cells
Duration: 2022 – 2024
Grant Agency of the Charles University, 358322 (M. Tulpa)
Title: Interaction of galectin-3 with the NKp30 receptor and its role in the antitumor immune response
Duration: 2022 – 2024
Grant Agency of the Charles University, 285423 (N. Bragina)
Title: Novel recombinant immunocytokines to stimulate the anti-tumour immune response
Duration: 2023 – 2025
Grant Agency of the Charles University, 173923 (M. Hejl)
Title: Utilising sortase A for the preparation of polymer immunotherapeutics and determining their antitumour activity
Duration: 2023 – 2025
Offered/Supervised/Defended theses
Publications
2022
Anderluh M., Berti F., Bzducha-Wróbel A., Chiodo F., Colombo C., Compostella F., Durlik K., Ferhati X., Holmdahl R., Jovanovic D., Kaca W., Lay L., Marinovic-Cincovic M., Marradi M., Ozil M., Polito L., Reina-Martin J. J., Reis C. A., Sackstein R., Silipo A., Švajger U., Vaněk O., Yamamoto F., Richichi B., van Vliet S. J. (2022): Recent advances on smart glycoconjugate vaccines in infections and cancer. FEBS J 289, 4251–4303. IF 5.622 DOI: 10.1111/febs.15909
Vaněk O., Kalousková B., Abreu C., Nejadebrahim S., Skořepa O. (2022): Natural killer cell-based strategies for immunotherapy of cancer. Adv Protein Chem Struct Biol 129, 91-133. IF 5.447 DOI: 10.1016/bs.apcsb.2022.02.001
Remans K., Lebendiker M., Abreu C., Maffei M., Sellathurai S., May M. M., Vaněk O., de Marco A. (2022): Protein purification strategies must consider downstream applications and individual biological characteristics. Microb Cell Fact 21, 52. IF 6.352 DOI: 10.1186/s12934-022-01778-5
Bláha J., Skálová T., Kalousková B., Skořepa O., Cmunt D., Grobárová V., Pazicky S., Poláchová E., Abreu C., Stránský J., Kovaľ T., Dušková J., Zhao Y., Harlos K., Hašek J., Dohnálek J., Vaněk O. (2022): Structure of the human NK cell NKR-P1:LLT1 receptor:ligand complex reveals clustering in the immune synapse. Nat Commun 13, 5022. IF 17.694 DOI: 10.1038/s41467-022-32577-6
2021
Stehlík Š., Henych J., Štenclová P., Král R., Zemenová P., Pangrác J., Vaněk O., Kromka A., Rezek B. (2021): Size and nitrogen inhomogeneity in detonation and laser synthesized primary nanodiamond particles revealed via salt-assisted deaggregation. Carbon 171, 230-239. IF 11.307 DOI: 10.1016/j.carbon.2020.09.026
Zouharová M., Vymětal J., Bednárová L., Vaněk O., Herman P., Vetýšková V., Poštulková K., Lingstaadas P. S., Vondrášek J., Boušová K. (2021): Intrinsically disordered protein domain of human ameloblastin in synthetic fusion with calmodulin increases calmodulin stability and modulates its function. Int J Biol Macromol 168, 1-12. IF 8.025 DOI: 10.1016/j.ijbiomac.2020.11.216
Stehlík Š., Mermoux M., Schummer B., Vaněk O., Kolářová K., Štenclová P., Vlk A., Ledinský M., Pfeifer R., Romanyuk O., Gordeev I., Roussel-Dherbey F., Němečková Z., Henych J., Bezdička P., Kromka A., Rezek B. (2021): Size effects on surface chemistry and Raman spectra of sub-5 nm oxidized high-pressure high-temperature and detonation nanodiamonds. J Phys Chem C 125, 5647-5669. IF 4.177 DOI: 10.1021/acs.jpcc.0c09190
Anderluh M., Berti F., Bzducha-Wróbel A., Chiodo F., Colombo C., Compostella F., Durlik K., Ferhati X., Holmdahl R., Jovanovic D., Kaca W., Lay L., Marinovic-Cincovic M., Marradi M., Ozil M., Polito L., Reina-Martin J. J., Reis C. A., Sackstein R., Silipo A., Švajger U., Vaněk O., Yamamoto F., Richichi B., van Vliet S. J. (2021): Emerging glyco-based strategies to steer immune responses. FEBS J 288, 4746-4772. IF 5.622 DOI: 10.1111/febs.15830
Peleg Y., Vincentelli R., Collins B. M., Chen K., Livingstone E. K., Weeratunga S., Leneva N., Guo Q., Remans K., Perez K., Bjerga G. E. K., Larsen Ø., Vaněk O., Skořepa O., Jacquemin S., Poterszman A., Kjaer S., Christodoulou E., Albeck S., Dym O., Ainbinder E., Unger T., Schuetz A., Matthes S., Bader M., de Marco A., Storici P., Semrau M. S., Stolt-Bergner P., Aigner C., Suppmann S., Goldenzweig A., Fleishman S. J. (2021): Community-wide experimental evaluation of the PROSS stability-design method. J Mol Biol 433, 166964. IF 6.151 DOI: 10.1016/j.jmb.2021.166964
López-Méndez B., Baron B., Brautigam C. A., Jowitt T. A., Knauer S. H., Uebel S., Williams M. A., Sedivy A., Abian O., Abreu C., Adamczyk M., Bal W., Berger S., Buell A. K., Carolis C., Daviter T., Fish A., Garcia-Alai M., Guenther C., Hamacek J., Holková J., Houser J., Johnson C., Kelly S., Leech A., Mas C., Matulis D., McLaughlin S. H., Montserret R., Nasreddine R., Nehmé R., Nguyen Q., Ortega-Alarcón D., Perez K., Pirc K., Piszczek G., Podobnik M., Rodrigo N., Rokov-Plavec J., Schaefer S., Sharpe T., Southall J., Staunton D., Tavares P., Vaněk O., Weyand M., Wu D. (2021): Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study. Eur Biophys J 50, 411-427. IF 2.095 DOI: 10.1016/10.1007/s00249-021-01532-6
Boušová K., Bednárová L., Zouharová M., Vetýšková V., Poštulková K., Hofbauerová K., Petrvalská O., Vaněk O., Tripsianes K., Vondrášek J. (2021): The order of PDZ3 and TrpCage in fusion chimeras determines their properties – a biophysical characterization. Protein Sci 30, 1653-1666. IF 6.993 DOI: 10.1002/pro.4107
Kalousková B., Skořepa O., Cmunt D., Abreu C., Krejčová K., Bláha J., Sieglová I., Král V., Fábry M., Pola R., Pechar M., Vaněk O. (2021): Tumor marker B7-H6 bound to the coiled coil peptide-polymer conjugate enables targeted therapy by activating human natural killer cells. Biomedicines 9, 1597. IF 4.757 DOI: 10.3390/biomedicines9111597
2020
Janisova L., Gruzinov A., Zaborova O. V., Velychkivska N., Vaněk O., Chytil P., Etrych T., Janoušková O., Zhang X., Blanchet C., Papadakis C. M., Svergun D. I., Filippov S. K. (2020): Molecular mechanisms of the interactions of N-(2-hydroxypropyl)methacrylamide copolymers designed for cancer therapy with the blood plasma proteins. Pharmaceutics 12, 106. IF 6.321 DOI: 10.3390/pharmaceutics12020106
Skořepa O., Pažický S., Kalousková B., Bláha J., Abreu C., Ječmen T., Rosůlek M., Fish A., Sedivy A., Harlos K., Dohnálek J., Skálová T., Vaněk O.* (2020): Natural killer cell activation receptor NKp30 oligomerization depends on its N-glycosylation. Cancers 12, 1998. IF 6.639 DOI: 10.3390/cancers12071998
Sumová P., Polanská N., Leštinová T., Spitzová T., Kalousková B., Vaněk O., Volf P., Rohoušová I. (2020): Phlebotomus perniciosus recombinant salivary proteins polarize murine macrophages towards the anti-inflammatory phenotype. Front Cell Infect Microbiol 10, 427. IF 5.293 DOI: 10.3389/fcimb.2020.00427
Vetýšková V., Zouharová M., Bednárová L., Vaněk O., Sázelová P., Kašička V., Vymětal J., Srp J., Rumlová M., Charnavets T., Poštulková K., Reseland J. E., Boušová K., Vondrášek J. (2020): Characterization of AMBN I and II isoforms and study of their Ca2+-binding properties. Int J Mol Sci 21, 9293. IF 5.923 DOI: 10.3390/ijms21239293
Skálová T., Lengálová A., Dohnálek J., Harlos K., Mihalčin P., Kolenko P., Stráňava M., Bláha J., Shimizu T., Martínková M. (2020): Disruption of the dimerization interface of the sensing domain in the dimeric heme-based oxygen sensor AfGcHK abolishes bacterial signal transduction. J Biol Chem 295, 1587-1597. IF 5,157 DOI: 10.1074/jbc.RA119.011574
2019
Sumová P., Šíma M., Kalousková B., Polanská N., Vaněk O., Oliveira F., Valenzuela J. G., Volf P. (2019): Amine-binding properties of salivary yellow-related proteins in phlebotomine sand flies. Insect Biochem Mol Biol 115, 103245. IF 3.827 DOI: 10.1016/j.ibmb.2019.103245
Willen L., Leštinová T., Kalousková B., Sumová P., Spitzová T., Velez R., Domenech E., Vaněk O., Gállego M., Mertens P., Volf P. (2019): Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis. PLoS Negl Trop Dis 13, e0007832. IF 3.885 DOI: 10.1371/journal.pntd.0007832
Vaněk O.*, Celadová P., Skořepa O., Bláha J., Kalousková B., Dvorská A., Poláchová E., Pucholtová H., Kavan D., Pompach P., Hofbauerová K., Kopecký V. Jr., Mesci A., Voigt S., Carlyle J. R. (2019): Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells. Sci Rep 9, 17836. IF 3.998 DOI: 10.1038/s41598-019-52114-8
2018
Škerlová J., Bláha J., Pachl P., Hofbauerová K., Kukačka Z., Man P., Pompach P., Novák P., Otwinowski Z., Brynda J., Vaněk O.*, Řezáčová P. (2018): Crystal structure of native β-N-acetylhexosaminidase isolated from Aspergillus oryzae sheds light onto its substrate specificity, high stability, and regulation by propeptide. FEBS J 285, 580-598. IF 4.739 DOI: 10.1111/febs.14360
Malý M., Skálová T., Švecová L., Dohnálek J., Bláha J., Vaněk O., Harlos K., Kolenko P. (2018): Paired refinement: Impact of reintegration and rescaling. In: Dragounová K., ed. Sborník 8. Studentské vědecké konference fyziky pevných látek a materiálů FJFI ČVUT. Praha: České vysoké učení technické v Praze, s. 8-10. ISBN 978-80-01-06511-2
2017
Bláha J., Kalousková B., Skořepa O., Pažický S., Novák P., Vaněk O.* (2017): High-level expression and purification of soluble form of human natural killer cell receptor NKR-P1 in HEK293S GnTI- cells. Protein Expr Purif 140, 36-43. IF 1.338 DOI: 10.1016/j.pep.2017.07.016
Stehlík Š., Varga M., Štenclová P., Ondič L., Ledinský M., Pangrác J., Vaněk O., Lipov J., Kromka A., Rezek B. (2017): Ultrathin Nanocrystalline Diamond Films with Silicon Vacancy Color Centers via Seeding by 2 nm Detonation Nanodiamonds. ACS Appl Mater Interfaces 9, 38842-38853. IF 8.097 DOI: 10.1021/acsami.7b14436
Stráňava M., Man P., Skálová T., Kolenko P., Bláha J., Fojtíková V., Martínek V., Dohnálek J., Lengálová A., Rosůlek M., Shimizu T., Martínková M. (2017): Coordination and redox state-dependent structural changes of the heme-based oxygen sensor AfGcHK associated with intraprotein signal transduction. J Biol Chem 292, 20921-20935. IF 4.011 DOI: 10.1074/jbc.M117.817023
2016
Doležal M., Zábranský A., Dostál J., Vaněk O., Brynda J., Lepšík M., Hadravová R., Pichová I. (2016): Myristoylation drives dimerization of matrix protein from mouse mammary tumor virus. Retrovirology 13, 2. IF 3.867 DOI: 10.1186/s12977-015-0235-8
Stráňava M., Martínek V., Man P., Fojtíková V., Kavan D., Vaněk O., Shimizu T., Martínková M. (2016): Structural Characterization of the Heme-based Oxygen Sensor, AfGcHK, its Interactions with the Cognate Response Regulator, and their Combined Mechanism of Action in a Bacterial Two-component Signaling System. Proteins 84, 1375-1389. IF 2.289 DOI: 10.1002/prot.25083
2015
Bláha J., Pachl P., Novák P., Vaněk O.* (2015): Expression and purification of soluble and stable ectodomain of natural killer cell receptor LLT1 through high-density transfection of suspension adapted HEK293S GnTI- cells. Protein Expr Purif 109, 7-13. IF 1.407 DOI: 10.1016/j.pep.2015.01.006
Skálová T., Bláha J., Harlos K., Dušková J., Kovaľ T., Stránský J., Hašek J., Vaněk O., Dohnálek J. (2015): Four crystal structures of human LLT1, a ligand for human NKR-P1, in varied glycosylation and oligomerization states. Acta Crystallogr Sect D Biol Crystallogr 71, 578-591. IF 2.512 DOI: 10.1107/S1399004714027928
Machová I., Snášel J., Dostál J., Brynda J., Fanfrlík J., Singh M., Tarábek J., Vaněk O., Bednárová L., Pichová I. (2015): Structural and functional studies of phosphoenolpyruvate carboxykinase from Mycobacterium tuberculosis. PLoS ONE 10, e0120682. IF 3.057 DOI: 10.1371/journal.pone.0120682
Lynn G. M., Laga R., Darrah P. A., Ishizuka A. S., Balaci A. J., Dulcey A. E., Pechar M., Pola R., Gerner M. Y., Yamamoto A., Buechler C. R., Quinn K. M., Smelkinson M. G., Vaněk O., Cawood R., Hills T., Vasalatiy O., Kastenmüller K., Francica J. R., Stutts L., Tom J. K., Ryu K. A., Esser-Kahn A. P., Etrych T., Fisher K. D., Seymour L. W., Seder R. A. (2015): In vivo characterization of the physicochemical properties of polymer-linked TLR agonists that enhance vaccine immunogenicity. Nat Biotechnol 33, 1201-1210. IF 43.113 DOI: 10.1038/nbt.3371
Collaboration
Academic partners
Dr. Mario Brameshuber, Technical University of Vienna, Austria
doc. Ario de Marco, University Nova Gorica, Slovenia
prof. James R. Carlyle, University Toronto, Canada
Dr. Jan Dohnálek, IBT CAS CR, BIOCEV
Dr. Jakub Tomala, IBT CAS CR, BIOCEV
Dr. Michal Pechar, IMC CAS CR
Dr. Jana Dvořáková, IMC CAS CR
Industry and commercial partners
Merck s.r.o. (2010-2018)
IOCB TECH s.r.o. (2017–2018)
PROGEN Biotechnik GmbH (2021)
Teaching (Department of Biochemistry, FoS CU)
MC250C17N – Advanced practical course in Biochemistry I
MC250C03 – Advanced practical course in biochemistry I for KATA
MC250C08 – Advanced practical course in biochemistry II
MC250P49 – Biology for biochemists
MC250P67 – Therapeutical proteins
MS750P02 – Introduction to knowledge and technology transfer
MC250P50 – Management of biochemistry
37 defended bachelor theses
19 defended master theses
3 defended PhD. theses
Alumni
Supervision of bachelor theses
Hájková Alena (2007): Preparation of vector for the recombinant expression of human protein NKR-P1, receptor of natural killer cell
Němcová Petra (2007): Preparation of vector for the recombinant expression of protein NKR-P1A, receptor of rat natural killer cell
Novotná Lenka (2008): Preparation of vector for the recombinant expression of protein NKR-P1B, receptor of rat natural killer cell
Celadová Petra (2008): Molecular cloning and amplification of b and g isoforms of Clr gene, ligand for Nkrp1 receptors of mouse NK cells
Holubová Martina (2008): Molecular cloning and amplification of B, D, and F isoforms of NKR-P1 mouse natural killer cell receptor
Bláha Jan (2010): Optimization of recombinant protein expression in HEK293 cell line
Čuperková Romana (2012): Optimization of Culture Medium for HEK293 Cell Line
Dvorská Anna (2012): Preparation and studies of complex of rat NK cell receptors NKR-P1B and Clrb
Leontovyč Adrian (2012): Preparation of expression vector for production of receptors CD69 and S1P1
Poláchová Edita (2012): Preparation of Expression Vectors That Regulate Cell Cycle and Apoptosis to Optimize Expression System of HEK293 Cell Line
Pucholtová Helena (2012): Preparation of dimeric form of mouse NK cell receptor NKR-P1C
Illéš David (2013): Optimization of purification of recombinant nitrilase from Arthroderma benhamiae
Mikulová Barbora (2013): Preparation of expression vectors for NKp65 and KACL, new members of human NK cell receptor family
Nový Jiří (2013): Preparation of the human NK cell receptor KACL
Petermannová Romana (2013): Production of auxin transporter PIN1 in HEK293 cell line
Slavata Lukáš (2013): Preparation of plasmids for expression of interleukin 2-fusion monoclonal antibody
Kalousková Barbora (2014): Preparation of glycosylated form of human immunoreceptor NKp30
Pažický Samuel (2014): Preparation of expression vectors for receptor NKp30 and its ligands B7-H6 and BAG-6
Skořepa Ondřej (2014): Preparation of lentiviral expression vector with reporter gene
Gáthy Lajos (2015): Recombinant expression and purification of C324S modified nitrilase from Arthroderma benhamiae
Prokopová Tereza (2015): Production and purification of recombinant receptor Clrb
Herynek Štěpán (2017): Recombinant preparation of proliferating cell nuclear antigen (PCNA)
Krejčová Kateřina (2017): Preparation of tumour ligand B7-H6 with coiled-coil tag and verifying of its binding to receptor NKp30
Hausleitner Filip (2018): The influence of S159A mutation on the oligomeric state of human NK cell receptor NKR-P1A
Houserová Jana (2018): Recombinant expression of protein NKp46 and its ligand Epa1
Nepokojová Tereza (2018): Preparation of fusion ligands and evaluation of their binding to NK cell receptors
Runová Alžběta (2018): Preparation of receptor AICL in fusion with Fc fragment of human IgG
Rožová Dominika (2020): Production of interleukin 2 in fusion with monoclonal antibody S4B6
Gabriela Chytrá (2021): Study of the role of N-glycosylation in function of anti-HER2 nanobody
Hejl Maxmilián (2021): Optimization of in vitro assays focused on activation of NK cells
Kozojedová Petra (2021): Preparation of reporter cell line for validation of recombinant protein biological activity
Pazderová Kristýna (2021): Mutagenesis and expression of protein NKp80, the activation receptor of human lymphocytes
Tulpa Matouš (2021): Galectin-3 and its interaction with the natural cytotoxicity receptor NKp30
Bragina Nadezda (2022): Production of recombinant substrates for transpeptidase reaction
Dubanych Yurii (2022): Production and characterization of galectin-3 mutant
Rudenko Anastassiya (2022): Recombinant expression of the mouse NK cell receptor NKR-P1C
Urbanová Zuzana (2022): Recombinant expression of rat NK cell receptor Clr-b in the presence of fluorinated analogues of monosaccharides
Hubáček Jakub
Jeníček Jakub
Supervision of master theses
Hájková Alena (2009): Recombinant expression and studies on receptor hNKR-P1
Němcová Petra (2009): Recombinant expression and studies on rNKR-P1A receptor
Celadová Petra (2010): Preparation of rat NK cell receptors using HEK293T expression system
Bláha Jan (2012): Preparation and study of human lymphocyte receptor LLT1
Dvorská Anna (2014): Structural biology of complex of rat NK cell receptors NKR-P1B and Clrb
Hradilová Iveta (2014): Preparation and characterization of cyanide hydratase from Aspergillus niger and nitrilase from Arthroderma benhamiae
Poláchová Edita (2014): Optimization of HEK293 cell line expression system by regulation of cell cycle and apoptosis
Pucholtová Helena (2014): Production of mouse NK cell receptor NKR-P1C and seeking of its ligand
Zawadová Dorota (2014): Recombinant expression and purification of nitrilase from Neurospora crassa
Nový Jiří (2015): Preparation and study of human NK cell receptor AICL
Petermannová Romana (2015): Preparation of HEK293 cell line expressing auxin transporter PIN7 and testing of inhibitors of auxin transport
Kalousková Barbora (2016): Preparation of human NK cell activation receptor NKp80 and its ligand AICL
Pažický Samuel (2016): Study of NKp30 oligomerization and its interaction with B7-H6
Skořepa Ondřej (2016): Structural studies of rat NK cell receptor NKR-P1B and its ligand Clrb
Abreu de Sousa Santos Celeste (2019): Interaction of Galectin-1 with human NK cell receptors
Cmunt Denis (2019): Preparation of fusion domains of human immunoreceptors for their utilization in immunotherapy
Herynek Štěpán (2019): Structural biology of receptor NKp44 and its ligand PCNA
Houserová Jana (2020): Study of the interaction of receptor NKp46 with adhesin Epa1
Frantová Eliška (2022): Production of IL-2 fusion antibodies and determination of their biological activity
Malá Viktorie (2022): Preparation of mutated forms of protein B7-H6
Bednaříková Kristýna
Otépka Tomáš
Chytrá Gabriela
Hejl Maxmilián
Pazderová Kristýna
Tulpa Matouš
Bragina Nadezda
Dubanych Yurii
Rudenko Anastassiya
Urbanová Zuzana
Supervision of PhD. theses
Bláha Jan (2017): Study of structure and interaction of human lymphocyte receptors
Skořepa Ondřej (2021): Structural biochemistry of human NK cell receptor complex NKp30 and B7-H6
Kalousková Barbora (2022): Harnessing soluble forms of NK cell receptors and their ligands for cancer immunotherapy
Abreu de Sousa Santos Celeste
Nejadebrahim Shiva
Document Actions