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Modulation of key biotransformation enzymes by xenobiotics

Research Topics

  • Interaction of food supplements with xenobiotic biotransformation enzymes
  • Effect of dihydromyricetin on ethanol metabolism
  • Study on oxidative damage on rat hepatocyte model
  • Interaction of gut microflora with selected flavonoids
  • Role of intestinal microbiota in drug and carcinogen metabolism
  • Interaction of  selected cosmetic additives with biotransformation enzymes

Staff

prof. Petr Hodek,  Bc. Štěpán Balatka

 

Topics of Bachelor and Diploma Thesis

The list of the thesis is available here

 

Contact

Department of Biochemistry, Faculty of Science, Charles University

Hlavova 2030/8, 128 43 Prague 2,  door 221, elevated second floor,

tel: +420 221 951 287

e-mail: hodek@natur.cuni.cz

 

Collaboration

  • Department of Pharmacology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

  • Center for Advanced Preclinical Imaging, 1st Faculty of Medicine, Charles University, Prague, Czech Republic

 

Grants

Centre of drug-dietary supplements interactions and nutrigenetics, 2012-2018 (GAČR P303/12/G163)

Study on structure-function relationships of cytochromes P450 - key enzymes involved in metabolism of drugs and activation of carcinogens, 2006-2008 (GAČR 303/06/0928)

 

Selected Papers

ŠULC, M., HODEK, P., STIBOROVÁ, M.; The binding affinity of carcinogenic N-nitrosodimethylamine and N-nitrosomethylaniline to cytochromes P450 2B4, 2E1 and 3A6 does not dictate the rate of their enzymatic N-demethylation. Gen. Physiol. Biophys., 29, 175-185 (2010).

HODEK, P., KŘÍŽKOVÁ, J., FREI, E., SINGH, R., ARLT, V. M., STIBOROVÁ, M.; Impact of beta-naphthoflavone on genotoxicity of food-derived carcinogens. Neuro Endocrinol. Lett., 32 (Suppl), 25-34 (2011).

NAIMAN, K., DRAČÍNSKÝ, M., HODEK, P., MARTÍNKOVÁ, M., SCHMEISER, H.H., FREI, E., STIBOROVA, M.; Formation, persistence, and identification of DNA adducts formed by the carcinogenic environmental pollutant o-anisidine in rats. Toxicol Sci., 127, 348-359 (2012)

HODEK, P., KOBLIHOVÁ, J., KIZEK, R., FREI, E., ARLT, V. M., STIBOROVÁ, M.; The relationship between DNA adduct formation by benzo[a]pyrene and expression of its activation enzyme cytochrome P450 1A1 in rat. Environ. Toxicol. Pharmacol., 36, 989-996 (2013).

STIBOROVÁ, J., MANHARTOVÁ, Z., HODEK, P., ADAM, V., KIZEK, R., FREI, E.; Formation of DNA adducts by ellipticine and its micellar form in rats - A comparative study. Sensors, 14, 22982-22997 (2014). 

HODEK, P., FOUSOVA, P., BRABENCOVA, E., MOSEROVA, M., PAVEK, P., ANZENBACHEROVA, E., BROTANEK, J., HUDECEK, J., FREI, E., STIBOROVA, M.; Effect of dihydromyricetin on benzo[a]pyrene activation in rats. Neuro Endocrinol. Lett., 35 (Suppl. 2), 158-168 (2014).

STIBOROVA, M., SCHMEISER, H.H., FREI, E., HODEK, P., MARTINEK, V.; Enzymes Oxidizing the Azo Dye 1-Phenylazo-2-Naphthol (Sudan I) and their Contribution to its Genotoxicity and Carcinogenicity. Curr. Drug. Metab., 15, 829-840 (2014). 

STIBOROVA, M., LEVOVA, K., BARTA, F., DRACINSKA, H., SULC, M., HODEK, P., FREI, E., ARLT, V. M., SCHMEISER, H. H.; Dicoumarol inhibits rat NAD(P)H:quinone oxidoreductase in vitro and induces its expression in vivoNeuro Endocrinol. Lett., 35 (Suppl. 2), 123-132 (2014).

STIBOROVÁ, M., BÁRTA, F., LEVOVÁ, K., HODEK, P., FREI, E., ARLT, V.M., SCHMEISER, H.H.: The influence of ochratoxin A on DNA adduct formation by the carcinogen aristolochic acid in rats. Arch. Toxicol. 89, 2141-2158 (2015). 

STIBOROVÁ, M., BÁRTA, F., LEVOVÁ, K., HODEK, P., SCHMEISER, H. H., ARLT, V. M., MARTÍNEK, V.; A mechanism of O-demethylation of aristolochic acid I by cytochromes P450 and their contributions to this reaction in human and rat livers: Experimental and theoretical approaches. Int. J. Mol. Sci., 16, 27561-27575 (2015). 

BARTA F., LEVOVA K., HODEK P., SCHMEISER H.H., ARLT V.M., STIBOROVA M.: The effects of heavy metal ions, phthalates and ochratoxin A on oxidation of carcinogenic aristolochic acid I causing Balkan endemic nephropathy. Neuro Endocrinol. Lett., 36 (Suppl. 1), 13–21 (2015). 

KOPEČNÁ-ZAPLETALOVÁ, M., KRASULOVÁ, K., ANZENBACHER, P., HODEK, P., ANZENBACHEROVÁ, E.: Interaction of isoflavonoids with human liver microsomal cytochromes P450: Inhibition of CYP enzyme activities. Xenobiotica, 47, 324-331 (2017). 

BEBOVÁ, M., BOŠTÍKOVÁ, Z., MOSEROVÁ, M., PÁVEK, P., ANZENBACHEROVÁ, E., STIBOROVÁ M., HODEK P.: Modulation of xenobiotic conjugation enzymes by dihydromyricetin in rats. Monatsh. Chem., 148, 2003–2009 (2017). 

MARTÍNEK, V., BÁRTA, F., HODEK, P., FREI, E., SCHMEISER, H.H., ARLT, V.M., STIBOROVÁ, M.: Comparison of the oxidation of carcinogenic aristolochic acid I and II by microsomal cytochromes P450 in vitro: experimental and theoretical approaches. Monatsh. Chem., 148, 1971-1981 (2017).

 

Teaching (Department of Biochemistry, FS CU)

Biochemistry I (MC250P03I, summer semester)

Applied Biochemistry  (MC250P51, summer semester)

Management of Biochemistry (MC250P50, summer semester)

Advanced Practical Course II (MC250C08, summer semester)

Clinical Biochemistry Practical Course  (MC250C44, fall semester)

 

Alumni

Jitka Křížková                     Mgr. 2006  

Zdenka Sobotková           Mgr. 2006

Kamila Burdová                Mgr. 2008

Martina Teplá                   Mgr. 2008

Lenka Pacholíková           Mgr. 2012

Iva Mácová                        Mgr. 2013

Eliška Brabencová           Mgr. 2013

Petra Fousová                   Mgr. 2014

Bebová Michaela             Mgr. 2014

Hrdinová Johana               Mgr. 2015

Dědič Jan                            Mgr. 2015

Zdislava Boštíková           Mgr. 2016

Petr Holý                            Mgr. 2017

Aneta Skotnicová             Mgr. 2019

Pavlína Hucková               Mgr. 2020

Gabriela Hauptmanová   Mgr. 2020

 

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