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Laboratory of Cystic Fibrosis Pathogenesis

Research

  • Preparation and characterization of chicken yolk antibody against virulence factors of bacteria Pseudomonas aeruginosa (PA) and complex Burkholderia cepacia (BC)
  • Development and testing of mouse model mimicking conditions of cystic fibrosis (CF) lungs
  • Effect of antibody or glycomimetics on the bacterium (PA, BC) adherence on epithelial cells ex vivo
  • Interaction of fluorescently labeled bacterial lectins with epithelium glycoconjugates on tissue slices of lungs from CF and non-CF subjects
  • Development of stable mRNA encoding the chloride channel CFTR for cell transfection
  • Determination of ion transport through CFTR channel in cell lines of airway epithelium 

 

Research group: Profylaxis of microbial infections in CF

Staff

prof. Petr Hodek, Mgr. Kubíčková, Mgr. Jana Rychnová, Bc. Patrik Pomazal, Kateřina Vyhnalová

 

 

Research group: Molecular basis of bacterial adherence in CF

Staff

Mgr. Kubíčková, prof. Petr Hodek, Mgr. Jana Rychnová, Bc. Patrik Pomazal, Kateřina Vyhnalová

 

 

 

Research group: Genetic repair of the CFTR defect 

Staff

 Dr. Bořek-Dohalská, prof. Petr Hodek

 

Topics of Bachelor and Diploma Thesis

The list of the thesis is available here

 

Contact

Department of Biochemistry, Faculty of Science, Charles University

Hlavova 2030/8, 128 43 Prague 2,  door 221, elevated second floor,

tel: +420 221 951 287

e-mail: hodek@natur.cuni.cz

 

Laboratory Introduction

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Collaboration

  • Department of Biochemistry, Faculty of Science,  Masaryk University, Brno, Czech Republic

  • Department of Pharmaceutical Chemistry, University of Debrecen, Debrecen, Hungary

  • Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic

  • Department of Pathology, 3rd Faculty of Medicine, Charles University, Prague, Czech Republic

  • Institute for Clinical and Experimental Medicine, Prague, Czech Republic

  • National Institute of Public Health, Prague, Czech Republic

  • Czech Cystic Fibrosis Association, Prague, Czech Republic

 

Selected Papers

THAI LE, S., MALINOVSKA, L., VAŠKOVÁ, M., MEZŐ, E., KELEMEN, V., BORBÁS, A., HODEK, P., WIMMEROVÁ, M., CSÁVÁS, M.: Investigation of the Binding Affinity of a Broad Array of l-Fucosides with Six Fucose-Specific Lectins of Bacterial and Fungal Origin. Molecules, 24, 2262 (2019).

KUBÍČKOVÁ, B., HADRABOVÁ, J., VAŠKOVÁ, L., MANDYS, V., STIBOROVÁ, M., HODEK, P.: Susceptibility of airways to Pseudomonas aeruginosa infection: mouse neuraminidase model; Monatsh. Chem., 148, 1993–2002 (2017).

VAŠKOVÁ, L., NOSKOVÁ, L., BLÁHOVÁ, B., WIMMEROVÁ, M., DŘEVÍNEK, P., KUBÍČKOVÁ, B., STIBOROVÁ, M., HODEK, P.; Evaluation of anti-PAIIL lectin hen yolk antibody as an agent inhibiting Pseudomonas aeruginosa adherence to epithelial cells. Monatsh. Chem.147, 889-896 (2016).

HADRABOVÁ, J., MAJEROVÁ, B., ČERNÁ, V., MOSEROVÁ, M., HOLUŠA, R., MANDYS, V., STIBOROVÁ, M., STŘÍŽ, I., HODEK, P.; Chicken immunoglobulins for prophylaxis: Effect of inhaled antibodies on inflammatory parameters in rat airways. J. Appl. Biomed., 13, 19-22 (2015).

NOSKOVÁ, L., KUBÍČKOVÁ, B., VAŠKOVÁ, L., BLÁHOVÁ, B., WIMMEROVÁ, M., STIBOROVÁ, M., HODEK, P.; Fluorescent cellular assay for screening agents inhibiting Pseudomonas aeruginosa adherence. Sensors, 15, 1945-1953 (2015).

HODEK, P., TREFIL, P., ŠIMŮNEK, J., HUDEČEK, J., STIBOROVÁ, M.; Optimized protocol of chicken antibody (IgY) purification providing electrophoretically homogenous preparations. Int. J. Electrochem. Sci., 8, 113-124 (2013).

 

Teaching (Department of Biochemistry, FS CU)

Biochemistry I (MC250P03I, summer semester)

Applied Biochemistry  (MC250P51, summer semester)

Management of Biochemistry (MC250P50, summer semester)

Advanced Practical Course II (MC250C08, summer semester)

Clinical Biochemistry Practical Course  (MC250C44, fall semester)

 

Alumni

Michaela Vašková              Mgr. 2019

Kateřina Dostálová            Mgr. 2019

Lucie Vašková                     Mgr. 2016

Arina Bolshova                   Bc. 2019

Hrdinová Johana                Mgr. 2015

Hadrabová Jana                  Mgr. 2015

Libuše Nosková                  Mgr. 2013

Barbora Majerová              Mgr. 2013

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