Chromatographic analytical methods

Assoc. Prof. Zuzana Bosáková, Ph.D.
(E-mail: bosakova@natur.cuni.cz)

• high performance liquid chromatography
• chiral separations
• microcolumn liquid chromatography – mass spectrometry
• monitoring of biologically important compounds in the environment
• garant of "Clinical and Toxicological Analysis" (KATA) – study

Prof. Pavel Coufal, Ph.D. (Head of the Team)
(E-mail: pcoufal@natur.cuni.cz)

• microseparation methods
• liquid chromatography
• capillary electrophoresis – mass spectrometry

Assoc. Prof. Radomír Čabala, Ph.D.
(E-mail: cabala@natur.cuni.cz)

• derivatization methods for gas chromatography
• gas and liquid chromatography
• mass spectrometry
• GC-MS, GC×GC-MS

Petr Kozlík, MSc.
(E-mail: petr.kozlik@natur.cuni.cz)

• mass spectrometry
• mass spectrometry connected to separation techniques (HPLC-MS)
• characterization of separation systems
• development and validation of separation methods
• forced degradation studies and stability testing of active pharmaceutical ingredients and dosage forms

Tomáš Křížek, Ph.D.
(E-mail: krizek@natur.cuni.cz)

• analysis of biological samples using nano-HPLC-MS/MS on chip and capillary electrophoresis
• development of method for determination of coenzymes in plant extracts
• development of electrophoretic determination of substrates a products of enzymes glycosidase
• development of contactless conductivity detection methods

Anna Kubíčková, Ph.D.
(E-mail: anna.kubickova@natur.cuni.cz)

• HPLC separations of ligands for biomedicine application of DOTA derivatives
• application of new generation columns (e.g. Purospher STAR or ZIC-HILIC columns specially destined for polar substances)
• development of postcolumn derivatization methods based on forming of complexes

Karel Nesměrák, Ph.D.
(E-mail: nesmerak@natur.cuni.cz)

• analytical determination of pharmaceutically and biologically important substances
• study of relationships between structure and properties of compounds
• electrochemical and spectrometric methods for study of physical-chemical properties of compounds

Jana Sobotníková (Suchánková), Ph.D.
(E-mail: jana.sobotnikova@natur.cuni.cz)

• high performance liquid chromatography, new stationary phases
• capillary liquid chromatography, capillary monolithic and packing columns for HPLC-MS
• separation of biologically active peptides