Structural biochemistry
and cell signaling
Laboratory of Structural Biology and Cell Signaling consists of a multidisciplinary team whose thematic focus is divided into two main directions. Structural biology group produces proteins by recombinant expression technologies and characterizes the protein or protein-ligand structures and their dynamics utilizing advanced mass spectrometric techniques. Cell signaling unit examines the linkages of cellular signaling to the metabolism of cancer cells using molecular biology and biochemistry methods. Through the collaboration of the two groups a unique research platform is formed, wherein the results obtained by the study of biological systems can also be verified and explained at the molecular level.
More information available at the laboratory website.
Focus
Structural mass spectrometry
– Method development
– Novel covalent labeling and cross-linking chemistry of proteins /nucleic acids
– Acid proteases as a tool for protein digestion
– Automation of HDX-MS and CX-MS workflow including software design
– Application to selected medicinally or biotechnologically important proteins with special focus on membrane proteins, dynamic and heavily modified proteins and their complexes
Ambient ion landing protein immobilization
– Surface Immuno-affinity substrates for clinical diagnostics
– Biochemically active plates for desorption mass spectrometry
Contact
Institute of Microbiology, CAS, v. v. i, Division BIOCEV
Průmyslová 595
252 50 Vestec
Czech Republic
tel: +420 325 873 610
e-mail: novakpe1@natur.cuni.cz
People
Petr Novak – head of the group
Petr Man – researcher
Petr Pompach – researcher
Josef Chmelik – researcher
Daniel Kavan – researcher
Teaching (Department of Biochemistry, Charles University)
Biochemické praktikum I – MC250C42N
Biochemické praktikum – MC250C31N
Biochemické praktikum pro CHŽP – MC250C30
Moderní metody výzkumu proteinů – MC250P21
Molekulární biologie a genetika I – MC250P15
Molekulární techniky – MC250P45
Proteomika a metody studia primární struktury biopolymerů – MC250P60N
Využití počítačů pro prezentace – MC250P07A
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