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Structure-functional proteomics of biomolecules

Contacts; Prezentation list ; People; Goals; Projects; Publications; Cooperations; Teaching; Alumni

News

Instalation of mikroHPLC Agilent Inf1290 (2019/10/21-23)

Successful repair of qTOF MS maXis PLUS turbopump (2019/10/11)

Roman receive GAUK project/financial support for azurin structure/functional study:-)

Tomáš is membre of new GAČR project in cooperation with KACh (Dr. Petr Kozlík)

Successful movo of ESI/APCI-qTOF MS maXis PLUS to joint lab with KACh (2018/11/14-16)

 

Contact

Katedra Biochemistry Faculty of Science Charles University

Hlavova 2030/8, 1. floor, door Num. 216

Phone: +420 221 951 281

E-mail: miroslav.sulc (at) natur.cuni.cz

 

People

Miroslav Šulc – Head

Tomáš Ječmen – junior member

Adriana Osičková - partial junior member

Renata Ptáčková - partial junior member

Aleš Ďuriš - Lab manager

Vladimír Ondruška – ext. PhD. student

Roman Tuzhilkin  – Mgr. student

Kristýna Barvíková  – Mgr. student (Cooperation with Dr. V. Vlachovou, FGU)

Anna Rychlá - Bc. student

Albert Málek – Bc. student

Terka – high school studena (SOČ)

 

GOALs

Strukture-functional mass spectrometry (MS)

  - The development of novel techniques

           -  Photo-Induced Cross-Linking

           - Photo-Induced Electron Release

                * Cupredoxin - azurin (P. aeruginosa)

           - Clinically relevant protein-protein interactions

MFO system - metabolism of xenobiotics

  - interaction of membrane proteins  (cytochrom b5, cytochrom P450, and their reduktases)

Bakterial pathogenesis

- Interaction of CyaA toxinu (B. pertusis)

- Interaction of Azurinu (P. aeruginosa)

Interaction of regulatory proteins

            * eukaryotic14-3-3

            * human calmodulin (CALcium MODULated protein)

 

Publications

2018

Osickova A, Balashova N, Masin J, Sulc M, Roderova J, Wald T, Brown AC, Koufos E, Chang EH, Giannakakis A, Lally ET, Osicka R.; Cytotoxic activity of Kingella kingae RtxA toxin depends on post-translational acylation of lysine residues and cholesterol binding. Emerg Microbes Infect. 2018 Nov 7;7(1):178. doi: 10.1038/s41426-018-0179-x.

Zahradník J, Kolenko P, Palyzová A, Černý J, Kolářová L, Kyslíková E, Marešová H, Grulich M, Nunvar J, Šulc M, Kyslík P, Schneider B.; The crystal structure of XdpB, the bacterial old yellow enzyme, in an FMN-free form. PLoS One. 2018 Apr 9;13(4):e0195299. doi: 10.1371/journal.pone.0195299.

2016

Šulc, M.; Indra, R.; Moserová, M.; Schmeiser, H.; Frei, E.; Arlt, V.; Stiborová, M.; The impact of individual cytochrome P450 enzymes on oxidative metabolism of benzo[a]pyrene in human livers. Environmental and Molecular Mutagenesis, 2016, Roč. 57, č. 3, s.229-235. IF 2.63 DOI: 10.1002/em.22001

Mrízová, I.; Moserová, M.; Milichovský, J.; Šulc, M.; Kizek, R.; Kopečková, K.; Arlt, V.; Stiborová, M.; Heterologous expression of human cytochrome P450 2S1 in Escherichia coli and investigation of its role in metabolism of benzo[a]pyrene and ellipticine. Monatshefte für Chemie, 2016, Roč. 147, č. 5, s.881-888. DOI: 10.1007/s00706-016-1738-2

Stiborová, M.; Indra, R.; Moserová, M.; Šulc, M.; Hodek, P.; Frei, E.; Schmeiser, H.; Arlt, V.; NADPH- and NADH-dependent metabolism of and DNA adduct formation by benzo[a]pyrene catalyzed with rat hepatic microsomes and cytochrome P450 1A1. Monatshefte für Chemie, 2016, Roč. 147, č. 5, s.847-855. DOI: 10.1007/s00706-016-1713-y

Wald, T.; Osičková, A.; Mašín, J.; Matyska Lišková, P.; Petry-Podgorska, I.; Matoušek, T.; Šebo, P.; Osička, R.; Transmembrane segments of complement receptor 3 do not participate in cytotoxic activities but determine receptor structure required for action of Bordetella adenylate cyclase toxin. Pathogens and Disease, 2016, Roč. 74, č. 3, s.nestránkováno. DOI: 10.1093/femspd/ftw008

Mašín, J.; Osičková, A.; Sukova, A.; Fišer, R.; Halada, P.; Bumba, L.; Linhartova, I.; Osička, R.; Šebo, P.; Negatively charged residues of the segment linking the enzyme and cytolysin moieties restrict the membrane-permeabilizing capacity of adenylate cyclase toxin. Scientific Reports, 2016, Roč. 6, č. SEP 1 2016, s.1-14. DOI: 10.1038/srep29137

Jirků, M.; Lánský, Z.; Bednárová, L.; Šulc, M.; Monincová, L.; Majer, P.; Vyklický, L.; Vondrášek, J.; Teisinger, J.; Boušová, K.; The characterization of a novel S100A1 binding site in the N-terminus of TRPM1. International Journal of Biochemistry and Cell Biology, 2016, Roč. 78, č. September 2016, s.186-193. DOI: 10.1016/j.biocel.2016.07.014

Maňásková-Postlerová, P.; Cozlová, N.; Dorosh, A.; Šulc, M.; Guyonnet, B.; Jonáková, V.; Acrosin inhibitor detection along the boar epididymis. International Journal of Biological Macromolecules, 2016, Roč. 82, č. January 2016, s.733-739. DOI: 10.1016/j.ijbiomac.2015.10.034

Šulc, M.; Mrízová, I.; Černá, T.; Frei, E.; Eckschlager, T.; Adam, V.; Kopečková, K.; Stiborová, M.; Effectiveness of human cytochrome P450 3A4 present in liposomal and microsomal nanoparticles in formation of covalent DNA adducts by ellipticine. Neuroendocrinology Letters, 2016, Roč. 37, č. Suppl. 1, s.95-102.

2015

Ječmen T, Ptáčková R, Černá V, Dračínská H, Hodek P, Stiborová M, Hudeček J, Šulc M.; Photo-initiated crosslinking extends mapping of the protein-protein interface to membrane-embedded portions of cytochromes P450 2B4 and b₅. Methods. 2015 Nov 1;89:128-37. doi: 10.1016/j.ymeth.2015.07.015.

Vimberg V, Kuzma M, Stodůlková E, Novák P, Bednárová L, Šulc M, Gažák R.; Hydnocarpin-Type Flavonolignans: Semisynthesis and Inhibitory Effects on Staphylococcus aureus Biofilm Formation. J Nat Prod. 2015 Aug 28;78(8):2095-103. doi: 10.1021/acs.jnatprod.5b00430.

Bousova K, Jirku M, Bumba L, Bednarova L, Sulc M, Franek M, Vyklicky L, Vondrasek J, Teisinger J.; PIP2 and PIP3 interact with N-terminus region of TRPM4 channel. Biophys Chem. 2015 Oct;205:24-32. doi: 10.1016/j.bpc.2015.06.004.

Zigo M, Dorosh A, Pohlová A, Jonáková V, Šulc M, Maňásková-Postlerová P.; Panel of monoclonal antibodies to sperm surface proteins as a tool for monitoring localization and identification of sperm-zona pellucida receptors. Cell Tissue Res. 2015 Mar;359(3):895-908. doi: 10.1007/s00441-014-2072-9.

 

Partnerships

Academic Labs

Proteome Centre, UVIC, Canada

Texas Medical School, University of Texas, Houston, USA

School Of Biological & Chemical Sciences, Queen Mary University of London, UK

Institute of Fyzical chemistry J. Heyrovského AV ČR, Praha

Institute of Microbiology  AV ČR, Praha

BIOCEV, Praha

Industry

Zentiva, a.s., CZ (2013–2014)

BEDO, Thailand, (2015-2016)

 

Teaching (Dept. of Biochemistry Charles Univ.)

MC250P51 - Applied biochemistry (LS, time requirement 2/0, 3 credits)

MC250P70 - Life - molekules and Biochemistry (ZS, rtime requirement 2/1, 3 credits)

MC250P08 - Biochemistry and biology of microorganisms (LS, time requirements 2/0, 3 credits)

MC250P09A - Biochemical Methods (ZS, time requirement 2/0, 3 credits)

MC250P09B - Methods for Studies of Biomolecules (ZS, time requirements 2/0, 4 credits)

MC250C09 - Practical Course in Biochemistry and Biology of Microorganisms (LS, time requirements 0/1, 1 credits)

MC250C31N - Practical course in biochemistry

MC250C32N - Practical course in biochemistry for teachers

MC250P40B - Biochemistry II (KATA)

MC250C23 - Biochemistry practise

MC250C33 - Biochemistry practise for teachers

16 - defended Bachelour Thesis

6 - defended Diploma Thesis

3 - defended PhD Thesis

1- SOČ práce

 

Alumni

Dominik Musil

Andrea Tichá

Zina Briki

David Landl

Radek Žídek

Kristýna Beňová

Kateřina Pešlová/Haladová

Martina Mazurová

Jana Štrohalmová

Antonín Knížek

Jiří Zahradník

Jana Michalová/Linhartová

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