E-mail | SIS | Moodle | Helpdesk | Libraries | cuni.cz | CIS More

česky | english Log in



Postdoctoral researchers – Translational control of the eukaryotic gene expression and Splicing in the regulation of gene expression in yeast

Postdoctoral researcher – Translational control of the eukaryotic gene expression:

Postdoctoral position is available for either a molecular biologist or a computational biologist (specialist in bioinformatics) to study a role of physical and functional interactions between translation initiation factors and 5’ and 3’ untranslated regions (UTRs) in a control of mRNA translation and stability. Both canonical and non-canonical translation initiation factors will be in a focus of the study. The main models currently employed in the laboratory are human cell lines (derived from acute leukemia), budding yeast, hepatitis C virus and the beetle Tribolium castaneum. A broad selection of models and used techniques allow us to ask questions ranging from those covering basic functions of translational apparatus to questions concerning associated evolutionary and developmental consequences. Deregulation of translation initiation and mRNA stability plays a key role in many human pathologies including cancer, leukemia and viral infection. We always try to expand our basic science projects to understanding of the corresponding diseases and thus we often co-operate with leading academic institutes and clinics.

Qualification and experience. We are looking for a highly motivated, enthusiastic and qualified researcher who would like to join our team and effort. If molecular biologists, candidates should have an experience either in RNA analyses and biochemistry or in cell biology techniques including confocal microscopy, life cell imaging and FRET. If computational biologists, candidates should have, besides basic knowledge of molecular genetics, strong background and interest in computational data analysis, experience with Linux/Unix shell, knowledge in database technologies and solid programming skills. Strong communication skills and the ability to interact and co-operate well with other scientists and students in the team are essential.

Lab website: http://web.natur.cuni.cz/~pospisek/, IRESite: http://www.iresite.org/

 

Postdoctoral Researcher – Splicing in the regulation of gene expression in yeast:

Postdoctoral position is available to study the regulation of splicing and the interplay between transcription and pre-mRNA processing in yeast. Candidates should be interested in the molecular mechanisms of gene expression regulation and should have experience in molecular genetics, biochemistry, RNA analyses and cell biology/microbiology.

The Laboratory is interested in the mechanisms of splicing regulation and the relationship between splicing and transcription using yeasts as model organisms. Both Saccharomyces cerevisiae and Schizosaccharomyces pombe are used to study evolutionarily conserved regulatory factors of the synthesis and maturation phases of transcript production. Introns in S. cerevisiae are relatively few and short; the spliceosomes still contain an almost complete array of splicing factors as compared to other organisms (S. pombe or higher eukaryotes). The study of splicing factors in an organism with reduced intronome should help to identify interactions that link splicing to other gene expression processes.

Potential aims include:

- Regulation of RNA helicases during the splicing cycle

- Factors and molecular mechanisms of coupling of transcription initiation, elongation, and splicing

- Mechanisms that coordinate transcript production & maturation with cell cycle progression

- Role and regulation of cyclophilins in the spliceosome of S. pombe

Position is already occupied

Document Actions