Methodology

• DNA immunization of mice by a gene gun
• Detection of immune reactions with ELISA and ELISPOT assays and intracellular staining of cytokines
• Izolation of cells from tumors and their characterization with flow cytometry
• Analysis of gene expression – RT-qPCR, immunobloting, immunofluorescent staining
• Gene cloning and modification by mutagenesis
• Inactivation of cellular genes with the CRISPR-Cas9 system

Laboratory Equippment

• Gene gun (Bio-Rad) pro biolistickou imunizaci myší DNA vakcínami
• Gene gun (Bio-Rad) for biolistic immunization of mice with DNA vaccines
• AutoMACS (Miltenyi Biotec) for magnetic separation of cells
• GentleMACS octo (Miltenyi Biotec) for tissue homogenization and preparation of single cells
• Equipment for cell cultures – laminar flow boxes, CO₂ incubators, centrifuges, microscopes
• Equipment for electrophoretic separation of DNA, RNA, and proteins – electrophoresis systems, power supplies, electroblotters
• etc.