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Methodology and Technical Support


  • DNA immunization of mice by a gene gun
  • Detection of immune reactions with ELISA and ELISPOT assays and intracellular staining of cytokines
  • Izolation of cells from tumors and their characterization with flow cytometry
  • Analysis of gene expression – RT-qPCR, immunobloting, immunofluorescent staining
  • Gene cloning and modification by mutagenesis
  • Inactivation of cellular genes with the CRISPR-Cas9 system

Laboratory Equippment

  • Gene gun (Bio-Rad) pro biolistickou imunizaci myší DNA vakcínami
  • Gene gun (Bio-Rad) for biolistic immunization of mice with DNA vaccines
  • AutoMACS (Miltenyi Biotec) for magnetic separation of cells
  • GentleMACS octo (Miltenyi Biotec) for tissue homogenization and preparation of single cells
  • Equipment for cell cultures – laminar flow boxes, CO2 incubators, centrifuges, microscopes
  • Equipment for electrophoretic separation of DNA, RNA, and proteins – electrophoresis systems, power supplies, electroblotters
  • etc.


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