Two positions for Ph.D. study open!

Topic: Study of cell interaction with nanoparticles with their application in bio-medicine.
Supervisor: Doc. RNDr. Marie Hubálek Kalbáčová, Ph.D., Biomedical Center,  Medical Faculty in Pilsen, Charles University

Study Form: full time study

Nanoparticles of different types have been widely used in bio-medicine in past several years; however their interactions with cells are still not well understood.  Nanoparticles used in this Project will be based on silicon and its composites and could be used for visualization within the cells as well as for drug/gene delivery. To fulfill this aim, deep study of nanoparticles entry into the cells, their movement and localization as well as possible escape from the cell is needed. To characterize the cell-nanoparticle interaction methods of molecular biology and biochemistry, confocal microscopy with subsequent image analysis as well as other physical methods will be used.

Topic: Study of cell interaction with nanoparticles based on viruses.
Supervisor: Doc. RNDr. Marie Hubálek Kalbáčová, Ph.D., Institute of Inherited Metabolic Disorders, 1st Faculty of Medicine, Charles University; marie.kalbacova@lf1.cuni.cz

Study Form: full time study

Mouse polyomavirus-based nanoparticles will be used as a modifiable tool for endocytosis study, which is important from the point of view of transport of different cargos (drugs, genes, fluorescence probes etc.) transported into cell by nanoparticles. To fulfill the aims of the project variably modified virus-based nanoparticles and broad spectrum of molecular biology and biochemistry methods, confocal microscopy with subsequent image analysis, electron microscopy as well as other physical methods will be used.

The project will be carried out in collaboration with the laboratory of virology, Faculty of Science, Charles University (supervisor: Dr. Hana Španielová, hs@natur.cuni.cz).