Lab of Molecular Anthropology

The Laboratory of Molecular Anthropology is part of the Department of Anthropology and Human Genetics at the Faculty of Science, Charles University. It provides technical background for research in the field of human genetics, molecular biology and immunology, particularly emphasizing clinical aspects and variability of human genome and immune response. The lab also guarantees and is in charge of lectures and practical courses regarding human genetics, molecular genetic and cytogenetic methods. Wide range of instrumentation can be used while doing research in the lab, including gradient PCR thermocyclers, bio-hazard boxes, Implen spectrophotometer, CO₂ incubator, vacuum centrifuge, cooled and conventional centrifuges, Carl Zeiss Primovert inverted microscope with AxioCam ERc 5s camera and ZEN 2011 software for image analysis, GelDoc XR gel documentation system with Image Lab v4.0 software for electrophoretic gel image analysis and other new equipment of the laboratory. Our lab offers research topics for theses of students in undergraduate, pregraduate and postgraduate study programmes.

The Laboratory of Molecular Anthropology also constitutes of the The Archaeogenetic Lab*, which is located at the Institute of Archaeology of the Czech Academy of Sciences.

Research Areas:
Archaeogenetics
Human Genetics and Clinical Genetics
Human Molecular Biology and Immunology/Immunogenetics

Lab Team

Head of the Lab:
RNDr. Pavlína Daňková, Ph.D.

Researchers:
*prof. Mgr. Viktor Černý, Dr.
RNDr. Roman Šolc, Ph.D.

PhD students:
Bhaswarupa Banerjee
Mgr. Iva Brynychová
*Mame Yoro Diallo, M.Sc.
*Mgr. Iva Kulichová
Mgr. Dominika Mravec Bencúrová
*Mgr. Eliška Podgorná
Mgr. Patrik Škubica

Pregradual students:
*Bc. Kristýna Adámková
Bc. Martina Beránková
*Bc. Tereza Holoubková
*Bc. Lívia Jurišová
Bc. Valeriia Kopytko
Bc. Katarína Selková

Cooperation

Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague
Institute of Rheumatology
Faculty Hospital Královské Vinohrady
Department of Physiology, Faculty of Science, Charles University
BIOCEV: Biotechnology and Biomedicine Center in Vestec 
Institute of Archaeology

Selected publications

Hudec, M., Dankova, P., Solc, R., Bettazova, N., & Cerna, M. (2020). Epigenetic Regulation of Circadian Rhythm and Its Possible Role in Diabetes Mellitus. International Journal of Molecular Sciences, 21(8), 3005.

Brynychová, I., Zinková, A., Hoffmanová, I., Korabečná, M., & Daňková, P. (2019). Immunoregulatory properties of cell-free DNA in plasma of celiac disease patients–A pilot study. Autoimmunity, 52(2), 88-94.

Heneberg, P., Kocková, L., Čecháková, M., Daňková, P., & Černá, M. (2018).  Autoimmunity-Associated PTPN22 Polymorphisms in Latent Autoimmune Diabetes of the Adult Differ from Those of Type 1 Diabetes Patients. International Archives of Allergy and Immunology ,177(1), 57–68.

Bohackova, E., & Dankova, P. (2017). Human Peripheral Blood Cells mRNA Levels are Highly Sensitive to Duration of Ex Vivo Post-Sampling Conditions Prior to RNA Isolation. Clinical Laboratory, 63(11), 1929–1933.

Zinkova, A., Brynychova, I., Svacina, A., Jirkovska, M., & Korabecna, M. (2017). Cell-free DNA from human plasma and serum differs in content of telomeric sequencies and its ability to promote immune response. Scientific Reports, 7(1), doi:10.1038/s41598-017-02905-8.

Hirschfeldova, K., & Solc, R. (2017). Comparison of SHOX and associated elements duplications distribution between patients (Lėri-Weill dyschondrosteosis/idiopathic short stature) and population sample. Gene, 627,164–168.

Solc, R., Klugerova, M., Vcelak, J., Baxova, A., Kuklik, M., Vseticka, J., Beharka, R., & Hirschfeldova, K. (2017). Mutation analysis of TRPS1 gene including core promoter, 5'UTR, and 3'UTR regulatory sequences with insight into their organization. Clinica Chimica Acta, 464, 30–36.

Herzogova, E., & Dankova, P. (2016). Ethylenediaminetetraacetic acid, sodium citrate, heparin and citrate phosphate dextrose-adenine anticoagulants differentially affect cytokine mRNA expression in blood leukocytes. Clinical Laboratory, 62(7), 1371–1374.

Brynychova, I., Hoffmanova, I., Dvorak, M., & Dankova, P. (2016). Increased Expression of TLR4 and TLR7 but Not Prolactin mRNA by Peripheral Blood Monocytes in Active Celiac Disease. Advances in Clinical and Experimental Medicine, 25(5), 887–893.

Cejkova, P., Nemeckova, I., Broz, J., & Cerna, M. (2016). TLR2 and TLR4 expression on CD14++ and CD14+ monocyte subtypes in adult-onset autoimmune diabetes. Biomedical Papers Med Fac Univ Palacky Olomouc Czech Repub.,160(1), 76–83

Solc, R., Hirschfeldova, K., Kebrdlova, V., & Baxova, A. (2014). Analysis of common SHOX gene sequence variants, and ~4.9kb PAR1 deletion in ISS patients. Journal of Genetics, 93(2), 505–508.

Cibulova, A., Zajacova, M., Fojtikova, M., Stolfa, J., Sedova, L., Cejkova, P., Lippert, J., Arenberger, P., & Cerna, M. (2013). The HLA-Cw*06 allele and -1149 G/T polymorphism of extrapituitary promoter of PRL gene as a possible common genetic predisposing factors to psoriasis vulgaris and psoriatic arthritis in Czech population. Rheumatology International, 33(4), 913–919.

Cejková, P., Chromá, V., Cerná, M., Marková, M., Marek, J., Lacinová, Z., & Haluzík, M. (2012). Monitoring of the course of sepsis in hematooncological patients by extrapituitary prolactin expression in peripheral blood monocytes. Physiological Research, 61(5), 481–488.

Hirschfeldova, K., Solc, R., Baxova, A., Zapletalova, J., Kebrdlova, V., Gaillyova, R., Prasilova, S., Soukalova, J., Mihalova, R., Lnenicka, P., Florianová, M., & Stekrova, J. (2012). SHOX gene defects and selected dysmorphic signs in patients of idiopathic short stature and Léri-Weill dyschondrosteosis. Gene, 491(2), 123–127.

Hirschfeldova, K., Baxova, A., Kebrdlova, V., Solc, R., Mihalova, R., Lnenicka, P., Vesela, K., Stekrova, J. (2011). Cryptic Chromosomal Rearrangements in Children with idiopathic mental retardation in the Czech population. Genetic Testing and Molecular Biomarkers, 15(9), 607–611.

Fojtíková, M., Novota, P., Čejková, P., Pešičková, S., Tegzová, D., & Černá, M. (2011). HLA class II, MICA and PRL gene polymorphisms: the common contribution to the systemic lupus erythematosus development in Czech population. Rheumatology International, 31(9), 1195–1201.

Fojtíková, M., Čejková, P., Bečvář, R., Vencovský, J., Tomasová-Studýnková, J., & Černá, M. (2010). Polymorphism of the extrapituitary prolactin promoter and systemic sclerosis. Rheumatology International, 30(12), 1691–1693.

Cejkova, P., Fojtikova, M., & Cerna, M. (2009). Immunomodulatory role of prolactin in diabetes development. Autoimmunity Reviews, 9, 23–27.

Cejkova, P., Novota, P., Cerna, M., Kolostova, K., Novakova, D., Novak, J., Ande,l M., & Zdarsky, E. (2008). HLA DRB1, DQB1 and insulin promoter VNTR polymorphisms: interactions and the association with adult-onset diabetes mellitus in Czech patiens. International Journal of Immunogenetics, 35(2), 133–140.

Fojtíková, M., Černá, M., Čejková, P., Růžičková, Š., & Dostál, C. (2007). Extrapituitary prolactin promoter polymorphism in Czech patients with systemic lupus erythematosus and rheumatoid arthritis. Annals of the Rheumatic Disease, 66 (5), 706–707.