Laboratoř vývojové biologie

V explozivně se rozvíjejícím oboru se laboratoř zaměřila na genetický a genomický přístup k embryologii u drápatky vodní (Xenopus laevis) a drápatky tropické (Xenopus tropicalis). V naší laboratoři se podařilo založit kulturu progenitorů Sertoliho buněk z varlat Xenopus tropicalis, u nichž studujeme jejich regenerační potenciál po poranění juvenilních i adultních tkání (ocas pulce a srdce dospělců). Ve spolupráci s Laboratoří imunoregulací (doc. Magdalena Krulová) se taktéž zaměřujeme na jejich imunomodulační schopnosti směrem k potlačení zánětlivé odpovědi a navození regenerativních procesů. Dále se zabýváme studiem diferenciačního potenciálu těchto kmenových buněk a schopností tvorby 3D organoidů semenotvorných kanálků in vitro. Pomocí fluorescenční in situ hybridizace (FISH) se snažíme přispět k poznání chromozomální evoluce u afrických pipovitých žab s využitím diploidního druhu Xenopus tropicalis a polyploidních druhů rodu Silurana a Xenopus. V neposlední řadě se naše skupina (ve spolupráci s Revmatologickým ústavem při 1. lékařské fakultě)

Adipogenní, chondrogenní a osteogenní diferenciace myších Sertoliho buněk (mSCs).

Karyotyp a tyramidová FISH chromozomů Xenopus tropicalis.

Mikroinjekce buněk XTSSC RFP do dorzální žíly pulce Xenopus tropicalis

Laboratoř:

Viničná 7, přízemí, č. dv. 40

Linka: 1773 (P40) , 1778 (P45), 1798 (P45)

Vedoucí skupiny:

Krylov Vladimír (Doc.Ing. RNDr., Ph.D.) - vedoucí skupiny
Kontaktní údaje: V7/P40; +420 22195 1773; vkrylov@natur.cuni.cz

Tlapáková Tereza (RNDr.,Ph.D)
Kontaktní údaje: V7/P40; +420 22195 1773; tereza.tlapakova@natur.cuni.cz

Postdok:

Knytl Martin (Ing., Ph.D.) - momentálně zahraniční stáž
Kontaktní údaje: V7/P40; +420 22195 1773; martin.knytl@natur.cuni.cz

Doktorandi:

Fornaini Nicola Reinaldo (Dr.)
Kontaktní údaje: V7/P45; +420 22195 1798; fornainni@natur.cuni.cz

Zhao Qing (MSc.)
Kontaktní údaje: V7/P45; +420 22195 1798; qing.zhao@natur.cuni.cz

Hynková Marie (Mgr.)

Kontaktní údaje: V7/P45; +420 22195 1798; marie.hynkova@natur.cuni.cz

Bergelová Barbora (Mgr.)

Kontaktní údaje: V7/P45; +420 22195 1798; barbora.bergelova@natur.cuni.cz

Magisterští studenti:

Novotná Jana (Bc.) - Email: jana.novotna@natur.cuni.cz

Šrámková Adéla (Bc.) - Email: adela.sramkova@natur.cuni.cz

Technik:

Vávra Jiří (Ing. Mgr.) - Kontaktní údaje: V7/P40, +420 22195 1773, vavraji1@natur.cuni.cz

Publikace 2021:

Song XY, Furman BLS, Premachandra T, Knytl M, Cauret CMS, Wasonga DV, Measey J, Dworkin I, Evans BJ (in press) Sex chromosome degeneration, turnover, and sex-biased expression of sex-linked transcripts in African clawed frogs (Xenopus) Philos Trans R Soc B-Biol Sci. DOI: 10.1098/rstb.2020.0095. IF₂₀₁₉ = 5.680.

Targueta CP, Krylov V, Nondilo TE, Lima J, Lourenço LB (2021) Sex chromosome evolution in frogs-helpful insights from chromosome painting in the genus Engystomops. Heredity 126(3):396-409. DOI: 10.1038/s41437-020-00385-7. IF₂₀₁₉ = 3.436.

Publikace 2020:

Sember A, Pelikanova S, de Bello Cioffi M, Slechtova V, Hatanaka T, Do Doan H, Knytl M, Rab P (2020) Taxonomic diversity not associated with gross karyotype differentiation: The case of bighead carps, genus Hypophthalmichthys (Teleostei, Cypriniformes, Xenocyprididae). Genes 11(5):479 IF₂₀₁₉ = 3.759.

Cauret CMS, Gansauge MT, Tupper AS, Furman BLS, Knytl M, Song XY, Greenbaum E, Meyer M, Evans BJ (2020) Developmental systems drift and the drivers of sex chromosome evolution. Mol Biol Evol 37(3):799-810. IF₂₀₁₉ = 11.062.

Furman BLS, Cauret CMS, Knytl M, Song XY, Pramachandra T, Ofori-Boateeng C, Jordan DC, Horb ME, Evans BJ (2020) A frog with three sex chromosomes that co-mingle together in nature: Xenopus tropicalis has a degenerate W and a Y that evolved from a Z chromosome. PLoS Genet. PLoS Genet 16(11): e1009121. IF₂₀₁₉ = 5.174.

Svaton M, Skvarova Kramarzova K, Kanderova V, Mancikova A, Smisek P, Jesina P, Krijt J, Stiburkova B, Dobrovolny R, Sokolova J, Bakardjieva-Mihaylova V, Vodickova E, Rackova M, Stuchly J, Kalina T, Stary J, Trka J, Fronkova E, Kozich V (2020). A homozygous deletion in the SLC19A1 gene as a cause of folate-dependent recurrent megaloblastic anemia. Blood. 135(26):2427-2431. DOI: 10.1182/blood.2019003178. IF₂₀₁₉ = 17.794.

Publikace 2019:

Nguyen TXM, Vegrichtova M, Tlapakova T, Krulova M, Krylov V. (2019) Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells. Stem Cells Int 5, 8387478 (IF = 3.989).

Nguyen TXM, Vegrichtova M, Tlapakova T, Krulova M, Krylov V. (2019) The interconnection between cytokeratin and cell membrane-bound β-catenin in Sertoli cells derived from juvenile Xenopus tropicalis testes. Biology open, submitted (IF = 2.217).

Stiburkova B, Bohata J, Minarikova I, Mancikova A, Vavra J, Krylov V, Doležel Z. (2019) Clinical and Functional Characterization of a Novel URAT1 Dysfunctional Variant in a Pediatric Patient with Renal Hypouricemia. Appl Sci 9, 3479 (IF = 2,217).

Toyoda Y, Mančíková A, Krylov V, Morimoto K, Pavelcová K, Bohatá J, Pavelka K, Pavlíková M, Suzuki H, Matsuo H, Takada T, Stiburkova B. (2019) Functional Characterization of Clinically-Relevant Rare Variants in ABCG2 Identified in a Gout and Hyperuricemia Cohort. Cells 8(4) (IF = 4.829)

Seroussi E, Knytl M, Pitel F, Elleder D, Krylov V, Leroux S, Morisson M, Yosefi S, Miyara S, Ganesan S, Ruzal M, Andersson L, Friedman-Einat M. (2019) Avian expression patterns and genomic mapping implicate leptin in digestion and TNF in immunity suggesting that their interacting adipokine role has been acquired only in mammals. Int J Mol Sci 20, 4489 (IF = 4,183).

Petelak A., Krylov V. (2019) Surface sperm cell ubiquitination directly impaired blastocyst formation rate after intracytoplasmic sperm injection in pig. Theriogenology 135, 115-120 (IF = 2.136).

Targueta CP, Krylov V, Nondilo TE, Lima J,  Lourenço LB. Sex chromosome evolution in frogs – helpful insights from chromosome painting in the genus Engystomops. Heredity, submitted. (IF = 3.872).

Publikace 2018:

Knytl M, Tlapakova T, Vankova T, Krylov V. (2018) Silurana chromosomal evolution: A new piece to the puzzle. Cytogenet Genome Res 156, 223-228 (IF = 1.587).

Knytl M, Kalous L, Rylková K, Choleva L, Merilä J, Ráb P. (2018) Morphologically indistinguishable hybrid Carassius female with 156 chromosomes: A threat for the threatened crucian carp, C. carassius, L. PLoS One 13, e0190924 (IF = 2.766).

Publikace 2017:

Knytl M, Smolik O, Kubickova S, Tlapakova T, Evans BJ, Krylov V (2017) Chromosome divergence during evolution of the tetraploid clawed frogs, Xenopus mellotropicalis and Xenopus epitropicalis as revealed by Zoo-FISH. PLoS One 12(5): e0177087. IF = 2.766.

Publikace (2013-2007)

Seifertova E, Zimmerman LB, Gilchrist MJ, Macha J, Kubickova S, Cernohorska H, Zarsky V, Owens ND, Sesay AK, Tlapakova T, Krylov V. (2013) Efficient high-throughput sequencing of a laser microdissected chromosome arm. BMC Genomics. 2013 May 28;14(1):357. (IF 4,397).

Bewick AJ, Chain FJ, Zimmerman LB, Sesay A, Gilchrist MJ, Owens ND, Seifertova E, Krylov V, Macha J, Tlapakova T, Kubickova S, Cernohorska H, Zarsky V, Evans BJ. (2013) A large pseudoautosomal region on the sex chromosomes of the frog Silurana tropicalis., Genome Biol Evol., in Press.

Flajnik MF, Tlapakova T , Criscitiello MF, Krylov V, Ohta Y. (2012) Evolution of the B7 family: co-evolution of B7H6 and NKp30, identification of a new B7 family member, B7H7, and of B7's historical relationship with the MHC.

Mácha J, Teichmanová R, Sater AK, Wells DE, Tlapáková T, Zimmerman LB, Krylov V. (2012) Deep ancestry of mammalian X chromosome revealed by comparison with the basal tetrapod Xenopus tropicalis. BMC Genomics 13:315. (IF=4.073)

Sanchez-Ramos I,Cross I,Mácha J, Martínez-Rodríguez,Krylov V,Rebordinos L. (2012) Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits. The ScientificWorld Journal, Volume 2012, Article ID 369802, 10 pages, doi:10.1100/2012/369802. (Impact factor coming in 2013)

Vackova I, Novakova Z, Krylov V, Okada K, Kott T, Fulka H, Motlik J. (2011) Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo. J Reprod Dev. 57(5):594-603. (bez afiliace)

Wells DE, Gutierrez L, Xu Z, Krylov V, Mácha J, Blankenburg KP, Hitchens M, Bellot L, Spivey M, Kowis A, Ye Y, Pasternak S, Owen J, Tran T, Slavíková R, Tůmová L, Tlapáková T, Seifertová E,Scherer SE, Sater AK (2011) A genetic map of Xenopus tropicalis. Dev Biol. 354(1): 1-8. (IF 4.379)

Zubáčová Z, Krylov V, Tachezy J. (2011) Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes. Mol Biochem Parasitol. 176(2):135-137. (IF 2.875)

Krylov V, Kubíčková S, Rubeš J, Mácha J, Tlapáková T, Seifertová E, Šebková N (2010) Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH. Chromosome Res18(4): 431-439. (IF 3.230)

Khokha M.K., Krylov V., Reilly M.J., Gall J.G., Bhattacharya D., Cheung C.Y., Kaufman S., Lam D.K., Macha J., Ngo C., Prakash N., Schmidt P., Tlapakova T., Trivedi T., Tumova L., Abu-Daya A., Geach T., Vendrell E., Ironfield H., Sinzelle L., Sater A.K., Wells D.E., Harland R.M., Zimmerman L.B. Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes. Dev Dyn. 2009; 238(6):1398-1346. (IF 2.833)

Krylov V, Kubíčková S, Rubeš J, Mácha J, Tlapáková T, Seifertová E, Šebková N (2010) Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH. Chromosome Res18(4): 431-439. (IF 3.230)

Khokha MK, Krylov V, Reilly MJ, Gall JG, Bhattacharya D, Cheung CY, Kaufman S, Lam DK, Mácha J, Ngo C, Prakash N, Schmidt P, Tlapáková T, Trivedi T, Tůmová L, Abu-Daya A, Geach T, Vendrell E, Ironfield H, Sinzelle L, Sater AK, Wells DE, Harland RM, Zimmerman LB (2009) Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes. Dev Dyn. 238(6):1398-46. (IF 2.864)

Krylov V, Tlapáková T, Mácha J, Curlej J, Ryban L, Chrenek P (2008) Localization of human coagulation factor VIII (hFVIII) in transgenic rabbit by FISH-TSA: identification of transgene copy number and transmission to the next generation. Folia Biol (Praha) 54(4):121-4. (IF 0.729)

Krylov V, Tlapakova T, Mácha J. (2007) Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA.(IF 1.783)