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Kill it, or get colonized?



Ing. Martin Janda, Ph.D.

(Ludwig-Maximilians-University of Munich (LMU), Genetics, Germany; University of Chemistry and Technology Prague (UCT Prague), Department of Biochemistry and Microbiology,  Czech Republic)


Kill it, or get colonized? That is the question which immunity help to solve for plants in response to ubiquitous microbes. But how to recognize if the microbe is „friend“ (symbiont) or „foe“ (pathogen)? And if it is pathogen how to block effectively its infection? And what strategies using pathogen to overcome plant immunity?
Here I will be focused on two distinct research topics within plant-pathogen interaction studied in our two projects:
i) What can trigger salicylic acid (SA) signalling pathway? SA is phytohormone playing a crucial role in plant response to pathogens. In our work, we show that its signalling pathway is triggered by several distinct events such as modulation of phospholipid signalling system1, disruption of actin cytoskeleton2 or treatment with saponin aescin3.
ii) How extracellular vesicles (EVs) of bacteria interact with plants? EVs are cytosol-containing membrane spheres that provide selection, storage and protection against degradation of enclosed cargoes in a highly dynamic and environmental cue-responsive manner. We recently found that the pathogen Pseudomonas syringae pv. tomato (Pto) DC3000 releases EVs in vitro and in planta. In plants EVs induced prototypic immune responses. Using proteomics, we determined the Pto DC3000 EV proteome. Interestingly, a large proportion of the EV proteins appears to be regulated in response to microbial pattern-triggered immunity (PTI).

1)Kalachova, T., Janda, M., et al.: Identification of salicylic acid-independent responses in an Arabidopsis phosphatidylinositol 4-kinase beta double mutant. (2019) Annals of botany. https://doi.org/10.1093/aob/mcz112
2)Leontovyčová H et al.: Actin depolymerization is able to increase plant resistance against pathogens via activation of salicylic acid signalling pathway. (2019) Scientific reports, doi: 10.1038/s41598-019-46465-5
3)Trdá, L., Janda, M., et al.: Dual Mode of the Saponin Aescin in Plant Protection: Antifungal Agent and Plant Defense Elicitor. (2019) Frontiers in Plant Science, doi: 10.3389/fpls.2019.01448

This research was supported by the European Structural and Investment Funds, OP RDE-funded project 'CHEMFELLS4UCTP' No. CZ.02.2.69/0.0/0.0/17_050/0008485 and by Czech Science Foundation GAČR grant no. 17-05151S.

CV of the speaker HERE.

Invitation to the talk HERE.

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