Biologická sekce
Přírodovědecké fakulty Univerzity Karlovy v Praze

Kontakty

Biologická sekce

Přírodovědecké fakulty UK

tajemnice:
Iva Hůleová
sekce-bi@natur.cuni.cz
22195 1600
Viničná 7, přízemí č.dv. 28

proděkan:

Doc. RNDr. Petr Folk, CSc.

4th Short Course on Time-Resolved Microscopy & Correlation Spectroscopy in Berlin-Adlershof

The course is intended for individuals wishing an in-depth introduction to the principles of time-resolved fluorescence microscopy and its applications to the Life Sciences. Attendees are typically professionals who are using or intend to use fluorescence microscopy in their research. Most attendees have some knowledge of fluorescence, typically in a specialized area. However, other individuals, from totally different research areas and industry, get the opportunity to enter this exciting field in a very effective way and benefit especially from the experimental section.
Čas 14.02.2012   08:15
16.02.2012   18:00
Jméno
Kontaktní telefon +493063926929
Přidat událost do kalendáře vKalendář (Windows, Linux)
iCal (Mac OS X)

Hands-on demonstration

Topics covered during the hands-on sessions include steady-state microscopy, FLIM, FCS, FCCS, FLCS, single molecule spectroscopy, (FLIM)-FRET and data analysis.

Participants will be divided into groups that rotate through the hands-on stations. This ensures that every participant will have the chance to familiarize himself with the broad range of techniques and methods of fluorescence (lifetime) microscopy. Please note that due to the expected number of participants, not every participant will have the possibility to work directly with the instruments.

The following companies will offer hands-on demonstration: (in alphabetical order)

Leica

  • TCS SP5 SMD - a confocal laser scanning microscope with integrated FLIM and FCS capabilities

Nikon

  • A1-R - a confocal laser scanning microscope, upgraded with FLIM and FCS capabilities

Olympus

  • FluoView FV1000 - a confocal laser scanning microscope, upgraded with FLIM and FCS capabilities

PicoQuant

  • MicroTime 200 - a time-resolved confocal fluorescence microscope
  • LSM Upgrade Kits - enable time-resolved measurements (FLIM, FCS, ...) with laser scanning microscopes

Time schedule (subject to changes)


Tuesday Wednesday Thursday
08:00 - 08:45
08:45 - 09:00
Registration
Welcome & Introduction


09:00 - 10:30 Christian Eggeling
Introduction to Microscopy
Mark Hink
Introduction to Fluorescence Correlation Spectroscopy
Jörg Enderlein
Advanced Fluorescence Correlation Spectroscopy
10:30 - 11:00 Coffee break Coffee break Coffee break
11:00 - 12:30 Rainer Erdmann
Instrumentation for Time-Resolved Microscopy
Paul French
Fluorescence Lifetime Imaging
Fred Wouters
Förster Resonance Energy Transfer
12:30 - 13:30 Group picture & Lunch break Lunch break Lunch break
13:30 - 15:30 Hands-on session 1 Hands-on session 3 Hands-on session 5
15:30 - 16:00 Coffee break Coffee break Coffee break
16:00 - 18:00 Hands-on session 2 Hands-on session 4 Hands-on session 6
18:00 - .... Opening reception

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